Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
互联网
| I. Exogenous Tracers | 
|---|
| 1. Markers of Protein Permeability | 
| Dyes: Evans blue | 
| Fluorescent Tracers: | 
| Fluorescein isothiocynate-dextran (MW: 62,000) | 
| Fluorescein isothiocynate-albumin (MW: 67,000) | 
| Horseradish peroxidasundefined (MW: 40,000) | 
| Microperoxidasundefined (MW: 1900) | 
| Radiolabeled Compounds | 
| 125Idine-labeled serum albumin | 
| 14 C-labeled dextran (MW: 70,000) | 
| 2. Markers of Solute and Ion Permeability | 
| Ionic Lanthanuundefined (MW: 138.9) | 
| Sodium Fluorescein (MW: 376) | 
| Lucifer Yellow (MW: 457) | 
| Ruthenium Reundefined (MW: 800) | 
| Fluorescein isothiocynate-dextran (MW: 3000) | 
| Radio-labeled Compounds | 
| 14 C-α -Aminoisobutyric acid (MW 103) | 
| 14 C Sucrose (MW: 342) | 
| 14 C- Methotrexate (MW: 455) | 
| 14 C-Inulin (MW: 5000) | 
| II.Endogenous Protein Extravasation | 
|---|
| Detected of Plasma Proteins by Immunohistochemistry | 
| Immunoperoxidase | 
| Immunofluorescence | 
| Immunogold technique | 











