Micropropagation of Rubus and Ribes spp.
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Micropropagation is the most appropriate method for large-scale production of Rubus and Ribes spp. The proliferation rate of Rubus spp. differs in shoot tips and nodal segments. The culture media used for raspberry and blackberry propagation are MS-based supplemented with different combination and ratio of plant growth regulators, depending on the stage of culture. The initiation medium containing 0.4 mg L−1 BA and 0.1 mg L−1 IBA is used to stabilize shoot cultures. In multiplication media, concentration of cytokinin is doubled. In vitro rooting of shoots is achieved on media supplemented with 1.0 mg L−1 IBA.
Ribes spp. cultures are initiated from shoot tips, meristem, or dormant buds on MS medium supplemented with 2.0 mg L−1 BA, 0.5 mg L−1 IBA, and 0.1 mg L−1 GA3. After stabilization of shoot cultures in 3–4-week time, shoot multiplication is carried out on MS medium containing 1.0 mg L−1 BA and 0.1 mg L−1 IBA. Shoots 2 cm long are cultured to rooting on a medium amended with 2.0 mg L−1 IBA and 5.0 mg L−1 IAA. Rooted plantlets are transferred to universal peat substrate and acclimatized in the greenhouse.
