• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Mass Measurement of Polyphosphoinositides by Thin-Layer and Gas Chromatography

        互联网

        597
        Phosphoinositides derive from the phospholipid, phosphatidylinositol (PtdIns), by phosphorylation of the inositol ring in the lipid head group. The determination of phosphoinositide species is a particular challenge, because the structurally similar inositolphosphate-head groups must be analyzed as well as the lipid-associated fatty acids. The method presented in this chapter consists of two steps: First phosphoinositides are separated by thin-layer chromatography (TLC) according to their characteristic head groups and the individual lipids are isolated. Second, the fatty acids associated with each isolated lipid are analyzed using a gas-chromatograph (GC). The combination of these two classical methods for lipid analysis, TLC and GC, provides a cost-efficient and reliable alternative to lipidomics approaches requiring more extensive instrumentation.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序