Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer
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Following the microinjection procedure (see Chapters 18 and 19 ), the eggs must be transferred to pseudopregnant (0.5 d pc) recipient mothers. Embryos from the one-cell through the morula stage (0.5–2.5 d pc) are transferred into the ampullae by a procedure called an oviduct transfer (1 ), described herein. The oviduct transfer is preferably performed on the same day as the microinjections or, alternatively, on the following day. The latter timing will allow the
