Molecular Typing Methods for Minor Histocompatibility Antigens

Minor histocompatibility (H) antigens crucially affect the outcome of human leukocyte antigen-matched allogenic stem cell transplantation. The number of molecularly identified minor H antigens is rapidly increasing. In parallel, clinical implementation of minor H antigens for immunotherapy has gained significant interest. It is therefore timely to type stem cell transplant recipients and their donors for minor H antigens. Here, we summarize all the currently known methodologies fro minor H antigen typing on the genomic and on the RNA level.