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        切除融合标签常用蛋白酶的酶切位点

        互联网

        26450

        Cleavage Sites Table for Fusion Proteins:

        PROTEASE
        COMPANY
        Protease Capture
        Thrombin
        Leu-Val-Pro-Arg�Gly-Ser
        Amersham-Biosciences, Novagen,
        SIGMA, Roche
        Benzamidine-Agarose
        Factor Xa
        Ile-Glu/Asp-Gly-Arg�
        Amersham-Biosciences, New England Biolabs,
        Roche
        Benzamidine-Agarose
        Enterokinase
        Asp-Asp-Asp-Asp-Lys�
        New England Biolabs,
        Novagen, Roche
        Trypsin Inhibitor-Agarose
        TEV protease
        Glu-Asn-Leu-Tyr-Phe-Gln�Gly
        Invitrogen – Life Technologies
        Ni-NTA (6His recomb. TEV)
        PreScission
        Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro
        Amersham-Biosciences
        GSTrap for GST fusion enzyme
        TAGZyme
        His-tag removal by Exoproteolytic Digestion
        Qiagen
        Ni-NTA (6His recomb. enzyme)
        Intein Site
        dithiothreitol cleavage
        New England Biolabs
        DTT elimination by dialysis
        HRV 3C Protease
        Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro
        Novagen
        Ni-NTA (6His recomb. enzyme)
        SUMO Protease
        recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO
        Invitrogen – Life Technologies, LifeSensors
        Ni-NTA (6His recomb. enzyme)

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