In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for rapid detection of phytoplasmas with levels of sensitivity and specificity approaching those of more complex and time-consuming laboratory methods.