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        P32放射自显影曝光时间 P32 Autoradiographic Exposure Times

        互联网

        1998
         
        Optimal exposure times must be empirically determined, but the following intervals are offered as a guide. In these cases, radioactive decay was measured on a Ludlum model 3 survey meter with a 44-3 detector, where 1 mR/hour is approximately 2500 counts per minute.

        For genomic DNA, cpm are usually at background. Expose a minimum of 4 days.
        For cloned DNA, use the following chart to estimate exposure times:

        CPM just above membrane Exposure Time
        < 0.2 K Overnight
        0.5 K 3-5 hours
        1 K 30 minutes
        3 K 20 minutes

        In the dark room, place a sheet of X-ray film on top of an intensifying screen, then place the hybridization membrane on top of the X-ray film facing the film. The hybridization membranes are pre-wrapped in cellophane. Close the film cassette, wrap it in foil to prevent spurious light leaks, label the outside and expose in a -70°C freezer.

        来自Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation
        http://omrf.ouhsc.edu/~frank/AUTORAD.html

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