Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes

互联网2014-02-13

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By directed differentiation using the hanging drop method, cardiomyocytes (CMs) can be derived from mouse embryonic stem cells. These spontaneously active CMs can then be isolated from the embryoid bodies and studied electrophysiologically for analysis of arrhythmic potential. This method is particularly advantangeous for studying CMs derived from genetically modified stem cells, in which mutations result in embryonic lethality.

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