Use of Fluorescent Proteins as Reporters

Fluorescent proteins (FPs) have established themselves as valuable reporter proteins in plant molecular biology. Beside general background information about spectral properties, protein structure and maturation of different commonly used FPs, this chapter provides detailed protocols about cloning of suitable expression cassettes for GFP and DsRED and detection of FPs in Arabidopsis protoplasts and plants transiently or stably expressing these constructs by fluorescence microscopy.