• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Assessments of Gliogenesis After Spinal Cord Injury

        互联网

        759
        Spinal cord injury (SCI) results in a large proliferative response that serves to restore homeostatis and replenish cellular deficits. Postinjury repair and recovery can be interrogated as a function of the cell fates adopted by progenitors within the lesion. Administration of bromodeoxyuridine (BrdU) (by ip injection) and/or a recombinant retrovirus (by intraspinal injection) is used routinely to label progenitors in an injured spinal cord. In combination with immunofluorescence, confocal microscopy assesses the progeny of labeled-progenitors. Colocalization of phenotypic markers with BrdU or retroviral-reporters is used to determine the differentiation profile of progenitors to assess gliogenesis after SCI. This chapter introduces a brief history of labeling proliferative cells, required materials and tools to phenotype progeny, typical procedures, and variations.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序