丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts

互联网

389
In vitro translation is a powerful method for specifically investigating mRNA translation independently of transcription, post-translational processing, and protein trafficking. There are several well-characterized in vitro translation systems including mammalian cell extracts (e.g., HeLa cells and Chinese Hamster Ovary cells) (1 ,2 ), rabbit reticulocyte lysates (3 ,4 ), Xenopus egg extracts (5 ), rye embryo (6 ), and wheat germ (7 ,8 ) that efficiently and faithfully translate heterologous mRNAs. Our laboratory is interested in investigating translational control of the angiotensin AT1 receptor. Thus, we need a cell-free system that efficiently translates the angiotensin AT1 receptor.
ad image
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序