General Preparation of Material and Staining of Sections

This chapter is aimed at those who have not previously done any processing for electron microscopy (EM). It deals with basic preparation of many different types of mammalian material for ultrastructural examination; for processing of plant material (see Hall and Hawes, ref. 1 ). The material to be processed may be cell suspensions, particulates, monolayer cultures, or tissue derived from organs. The former three must initially be processed differently from the latter.