• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase

        互联网

        604
        Mammalian cell lines expressing a recombinant gene of interest are conventionally generated by random genomic integration of exogenous DNA containing the recombinant gene and a selectable marker. In contrast, site-specific recombination targets the exogenous DNA to a preselected site in the mammalian genome (1 ,2 ). Genomic targeting with Cre recombinase of phage P1 is relatively simple and results in reproducible gene expression from a single copy of the inserted DNA (3 ). Thus, recombinase-mediated targeting can greatly speed the generation of stably transfected cell lines expressing a desired recombinant gene product. Because the cell lines obtained by Cre-mediated targeting are isogenic, one can eliminate the extensive functional screening required in conventional methods because of random integration and concomitant position and copy number effects on gene expression (4 ). Precise single-copy gene delivery to a defined chromosomal position is particularly valuable to characterize mutant regulatory sequences driving a reporter gene or to evaluate mutant alleles and variant isoforms of a particular recombinant gene, as has been done, for example, with the angiotensin AT1 receptor (5 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序