Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae
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This chapter describes methods for targeted knockouts using marker exchange mutagenesis and complementation of the Gram-negative bacteria Xanthomonas oryzae pv. oryzae . We have used these methods to demonstrate that type I secretion and modification systems are involved in avrXa21 activity of X. oryzae pv. oryzae .
