Studying the Influence of Salts on Partitioning of Proteins: Isoelectric Point Determination
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In addition to its application as a separation process, aqueous two-phase partitioning often yields information on important physical properties of proteins, replacing other more cumbersome analytical techniques. For example, aqueous two-phase partitioning can be used to measure the hydrophobicity and charge of a protein (1 -3 ), to calculate dissociation constants between enzymes and substrates (4 ), to fractionate cell populations (5 ), or to characterize cell surfaces (6 ,7 ).
