Blank control(blue): 293T(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Nuclear Pore Complex Proteins/Nup107/AF488 Conjugated antibody (bs-11678R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
- ￥1180 - 2800
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- bs-11678R
- 2026年10月14日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11678R |
| 英文名称 | NUP107 Rabbit pAb |
| 中文名称 | 核孔复合体蛋白抗体 |
| 英文别名 | 107 kDa nucleoporin; NPC proteins; NU107_HUMAN; Nuclear pore complex protein Nup107; Nucleoporin 107kDa; Nucleoporin Nup107; NUP 107; NUP 84; NUP84. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=3μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Nuclear Pore Complex Proteins |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 106 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Nuclear Pore Complex Signal Transduction > Protein Trafficking > Nuclear Import / Export Tags & Cell Markers > Subcellular Markers > Nucleus > Nuclear Pore |
| 亚基 | Forms part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. |
| 亚细胞定位 | Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Note=Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the nucleoporin Nup84/Nup107 family. |
| 功能 | Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {3μg/Test} |
Sample:
_x000D_ 293T Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (bs-11678R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 106kD
_x000D_ Observed band size: 106kD
_x000D_ 293T Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (bs-11678R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 106kD
_x000D_ Observed band size: 106kD
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文献和实验该产品被引用文献
[IF=5.182] Wei Lv. et al. Demonstration of intracellular trafficking, cytosolic bioavailability, and target manipulation of an antibody delivery platform. Nanomed-Nanotechnol. 2021 Feb;32:102315 IF Human
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NUP107 | NUP84 Rabbit pAb(bs-11678R)-50ul/100ul/200ul
￥1180 - 2800
