高速逆流色谱研究进展

摘　要：综述了近年来高速逆流色谱(HSCCC)在分析、半制备和制备分离天然产物、蛋白质、抗生素、无机物等领域的研究和应用进展，总结了适用于HSCCC的溶剂体系，并展望了HSCCC与质谱联用、pH区带逆流色谱和离子对逆流色谱新技术的应用前景。

关键词：高速逆流色谱，溶剂选择，天然产物，抗生素，蛋白质，无机物，质谱，pH区带逆流色谱，离子对逆流色谱，评述

分类号：O65　文献标识码：A

Recent Development in High-speed Countercurrent Chromatography

Dai Deshun（Analysis Center,Tsinghua University,Beijing 100084）　
Wang Yiming（Analysis Center,Tsinghua University,Beijing 100084）　
Luo Guoan（Analysis Center,Tsinghua University,Beijing 100084）　

Abstract：This paper reviews recent development and applications of high-speed countercurrent chromatography (HSCCC) in the separations on the analytical scale,the semi-preparative and preparative scale of natural products,antibiotics,proteins,inorganic compounds etc.Several series of two-phase solvent systems to facilitate the systematic search suitable for HSCCC are introduced.And the new countercurrent chromatography (CCC)technologies such as HSCCC coupled with MS,pH-zone-refining countercurrent chromatography,and ion-pairing Countercurrent Chromatography are explained and prospected in term of applications.
Keywords：High-speed countercurrent chromatography,solvent selection,natural products,antibiotics,proteins,inorganic compounds,mass spectrometry,pH-zone-refining countercurrent chromatography,ion-pairing countercurrent chromatography,review

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收稿日期：2000年4月17日

修稿日期：2000年10月17日

出版日期：2001年5月20日