实验动物的体重和周龄

This experiment allows you to observe the development of a chick embryo outside its shell. Cultured chicks are also more accessible for manipulation. Compare the extent of bone mineralization of contr ...

This is an easy method for stianing cartilage that can be used with isolated or CAM-grafted limbs to study limb development or with whole embryos (8 to 12 days of incubation ).1. Fix limbs or embryos ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl FGFs are implicated in mediating epithelial-mesenchymal interactions by means of regula ...

ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chick system will allow observation of general cartila ...

Abstract Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive events of limb formation. Experimenters have the ability to isolate and culture tissues outs ...

The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in great detail is the circulatory system. In the deve ...

Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioallantoic membrane (CAM) of chick embryos. Background ...

Abstract:Sonic Hedgehog is an essential glycoprotein integral for the proper craniofacial and tail bud development in addition to the development of many other structures. Cyclopamine has been shown t ...

ObjectiveThe purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treating them with five different concentrations of caff ...

Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish Aequorea victoria. In A.victoria the 27-kDa protein absorbs blue light from a photoprotein tha ...

Early in embryonic development the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. The next step in the formation of the actual limb (w ...

More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette smoke has long been associated with increased heal ...

ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with nicotine at early stages of development.Introducti ...

Put 500 mL of distilled water in a 1-L beaker. Add the following: Inorganic salts Muraschige�Skoog Organic medium � 34.6 g NaH2PO4H2O (stock 17 g/L) � 10-mL stock Organic constituents ucrose (6 % fina ...

蚊子是许多哺乳动物病原的宿主，包括寄生虫、细菌、病毒和真菌。疟疾主要是通过按蚊传播的疾病。为了控制疾病，了解传播的过程非常重要，因此需要研究蚊子幼虫的环境适应性。蚊子幼虫能够适应多变环境是因为蚊子的直肠有一个高度发育的离子调控系统。直肠负责吸收离子和营养，排出过量的盐和废弃物。但是这种调节的过程一直不清楚。 2010年，美国佛罗里达大学和MBL的科学家Smith等人使用非损伤微测技术等方法研究了按 ...

IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol one of several teratogenic agents which adversely affect the developing embryo. FAS is one of the mos ...

1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...

ABSTRACT Knowledge of the geneological relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Geneological relationships ca ...

Procedures for In Vitro Production of Bovine Embryos The procedures for in vitro production in vitro fertilization (IVF) and in vitro culture (IVC) of embryos described here are based on procedures d ...