原代培养

前言动物细胞培养开始于本世纪初 1962 年 其规模开始扩大 发展至今已成为生物、医学研究和应用中广泛采用的技术方法 利用动物细胞培养生产具有重要医用价值的酶、生长因子、疫苗和单抗等 已成为医药生物高技术产业的重要部分。利用动物细胞培养技术生产的生物制品已占世界生物高技术产品市场份额的50% 。 动物细胞大规模培养技术是生物技术制药中非常重要的环节。目前 动物细胞有悬浮培养和贴壁培养. ...

一、培养方法 如下图所示即为大鼠原代足细胞 按顺序：1，2是培养7天传代前的图片，小球周围爬出很多细胞。3，4是传代第二天细胞情况，过筛后仍有小的组织虑过。5，6是传代7天足细胞完全分化图片。 由于本人实验刚有进展，好多图片制作不理想，后续的细胞免疫荧光坚定在做，失败过两次，出结果后再发，不过老板说了就是足细胞！1 2345 过 ...

培养了快一个月的细胞了，今天终于鉴定出是我想要的血管平滑肌细胞。 我养的是大鼠胸主动脉血管平滑肌细胞采用的是组织贴块法培养。10天后传第一代，16天后第二代，22天后第三代，并做a－actin免疫细胞化学鉴定。附图片。 ...

ACDP Advisory Committee on Dangerous Pathogens BSA Bovine Serum albumin BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhoea virus FBS Fetal bovine serum EC European Community ECACC ...

1.Basic Techniques - The “Do’s and Don'ts” of Cell Culture Given below are a few of the essential "do’s and don’ts" of cell culture. Some of these are mandatory ...

1.Cell Culture Scale-up Systems Most tissue culture is performed on a small scale where relatively small numbers of cells are required for experiments. At this scale cells are usually grown in T flask ...

1.Authentication Techniques Whatever the scope of work to be carried out it is important to know that the work is being conducted using the correct reagents. This is no less important for cell culture ...

Introduction Quality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from ...

It is bad practice to maintain a cell line in continuous or extended culture for the following reasons:~undefined Risk of microbial contamination ~undefinedLoss of characteristics of interest (i.e. surface antigen or ...

1.Cryopreservation of Cell Lines The aim of cryopreservation is to enable stocks of cells to be stored to prevent the need to have all cell lines in culture at all times. It is invaluable when dealing ...

In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. Over the last t ...

1.Primary Cultures Primary cultures are derived directly from excised normal animal tissue and cultured either as an explant culture or following dissociation into a single cell suspension by enzyme d ...

Large numbers of cell lines look identical. Cell lines with very different origins and biological characteristics typically cannot be separated on grounds of morphology or culture characteristics. Inf ...

1.Risk Assessment The main aim of risk assessment is to prevent injury protect property and avoid harm to individuals and the environment. The performance of risk assessment is a legal requirement und ...

1.Laboratory Design Perhaps one of the most under-rated aspects of tissue culture is the need to design the facility to ensure that good quality material is produced in a safe and efficient manner. Mo ...

ECACC and Human Genetic Research The European Collection of Cell Cultures (ECACC) was first established within CAMR Salisbury UK in 1984 and has grown to be one of the largest animal cell culture coll ...

细胞培养基本概念 细胞培养是指从体内组织取出细胞在体外模拟体内环境下，使其生长繁殖，并维持其结构和功能的一种培养技术。细胞培养的培养物可以是单个细胞，也可以是细胞群。 细胞培养目的与用途 1、科学研究：药物研究开发与基础研究 药物研究与开发 (1) 新药筛选：如化学合成药物药效研究、中药有效成分筛选与鉴定等。 (2) 疫苗研究与开发：如病毒性疫苗的研究与开发（肝炎病毒疫苗、艾滋病疫苗等）、肿瘤疫苗 ...

无菌操作技术 体外培养细胞缺乏抗感染能力，所以防止污染是决定培养成功或失败的首要条件。即便使用设备完善的实验室，若实验者粗心大意，技术操作不规范也会导致污染。因而为在一切操作中最大可能地保证无菌，每一项工作都必须做到有条不紊和完全可靠。 Terms to remember 消毒(disinfection)和消毒剂(disinfectant) 灭菌(sterilization) 防腐(antis ...

Marc 145细胞培养和维持有关资料综述 一、 细胞及培养 1、Marc145细胞 上皮样细胞，来源于猴肾细胞，从母细胞（MA 104细胞）克隆而得到，可连续培养。 2、生长培养基 DMEM（高糖型，含4500mg/L D－葡萄糖、L-谷氨酰胺，和110mg/L丙酮酸钠，不含碳酸氢钠）＋5－10％新生牛血清+ 1%双抗（青霉素和链霉素）的培养液。 3、冻存液 生长培养基＋10% DMSO 4、细 ...

HepG2细胞培养的相关条件 HepG2人肝肿瘤细胞株广泛应用于遗传毒理学及病毒培养等方面的研究研究肝脏疾病发病机理及其临床应用有着重要意义。由于其与肝细胞具有相同的生物学活性，还被用于胰岛素抵抗的研究。本文对肝癌细胞培养的最佳条件做一简单综述。 1.HepG2细胞的复苏条件 1.1 复苏温度的选择 唐孟萱等采用下述方法进行细胞复苏：快速将所冻存细胞40℃水浴摇床60转/ min慢摇至其溶解，溶解 ...