siRNA制备实验

1 前言 RNA 是生物体内最重要的物质基础之一，它与 DNA 和蛋白质一起构成生命的框架。但长久以来， RNA 分子一直被认为是小角色。它从 DNA 那儿获得自己的顺序，然后将遗传信息转化成蛋白质。然而，一系列发现表明――这些小分子 RNA 事实上操纵着许多细胞功能。它可通过互补序列的结合反作用于 DNA ，从而关闭或调节基因的表达。甚至某些小分子 RNA 可以通过指导基因的开关来调 ...

Helvetica sans-serif" size="-1"Our current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western blotting. This allows us to test ...

Supplementary information to miRNAs cloned from mouse and human An online clearinghouse for miRNA gene name assignments (http://www.sanger.ac.uk/Software/Rfam/mirna/) is provided by the Rfam database ...

Here is our T7 siRNA protocol. The main idea is to design primers that begin with GG so that transcription by T7 polymerase is efficient. I) FIRST design oligos: Using sense coding sequence of any gen ...

Selection of siRNA duplexes from the target mRNA sequence Using Drosophila melanogaster lysates (Tuschl et al. 1999) we have systematically analyzed the silencing efficiency of siRNA duplexes as a fun ...