结构基因组学

Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)Day 11. Add formaldehyde directly to tissue culture media to a final concentration of 1%. We generally use 1 x 107 cells per anti ...

Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)Day 11. Add formaldehyde directly to tissue culture media to a final concentration of 1%. We generally use 1 x 107 cells per anti ...

Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial an ...

Mannie Liscum(1) and Paul Oeller(2)Department of Plant BiologyCarnegie Institution of WashingtonStanford CA 94305(1)Current address:http://www.biosci.missouri.edu/liscum/LiscumLabPage.html (site conta ...

Mannie Liscum(1) and Paul Oeller(2)Department of Plant BiologyCarnegie Institution of WashingtonStanford CA 94305(1)Current address:http://www.biosci.missouri.edu/liscum/LiscumLabPage.html (site conta ...

RLCS (Restriction Landmark cDNA Scanning) Protocolfor inmature barley grains(probably applicable for other plant materials)RLCS that was developed by Suzuki et al. (1996) displays many cDNA species q ...

RLCS (Restriction Landmark cDNA Scanning) Protocolfor inmature barley grains(probably applicable for other plant materials)RLCS that was developed by Suzuki et al. (1996) displays many cDNA species q ...

Microsatellite markers: isolation with non-radioactive probes and amplification Arnaud Estoup and Julie TurgeonThe main goal of this technical document (63 pages) is to provide geneticians and ecolo ...

b-galactosidase Solution AssayReference: Simon and Lis (1987) NAR 15: 2971-2988.SolutionsCPRG Assay Buffer50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.51 mM MgCl2 0.1 ml 1M MgCl2up to 100 ml with Qstore at r ...

b-galactosidase Solution AssayReference: Simon and Lis (1987) NAR 15: 2971-2988.SolutionsCPRG Assay Buffer50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.51 mM MgCl2 0.1 ml 1M MgCl2up to 100 ml with Qstore at r ...

ES injection pipette manufactureThe following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World P ...

Intracytoplasmic Spermatocyte Injection (ICSI) The number of infertile couples has increased in recent years and is now estimated at 15�20% of all couples worldwide. Since its introduction into repro ...

Bacterial beta-Galactosidase Histochemisty BibleThis is a collection of procedures and lore associated with staining mammalian tissue for bacterial beta-galactosidase. Unfortunately I can't attribute ...

Lac-Z Detection in Tail Biopsies2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X ...

Lac-Z Detection in Tail Biopsies2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X ...

Transgenic Animals The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in th ...

Transgenic Animals The term “transgenic animals” describes animals whose chromosomes contain stably integrated copies of genes or gene constructs derived from other species or not normally found in th ...

Methylated CpG island AmplificationProtocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI XmaIT4 DNA ligaseTaq DNA polymerase10X PCR reaction buffer:670mM Tris-HCl pH 8.840mM M ...

Methylated CpG island AmplificationProtocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI XmaIT4 DNA ligaseTaq DNA polymerase10X PCR reaction buffer:670mM Tris-HCl pH 8.840mM M ...

The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive research of methylation processes and it's influe ...