结构基因组学

This web site was designed to provide detailed protocols for genetic footprinting a technique for high-resolution mapping of the functional organization of cloned genes (Singh Crowley and Brown (1997) ...

Materials:Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media). Col ...

Materials:Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium #41675-018 These bottles usually come in groups of 10 (each bottle conmtaining 5 mL of media). Col ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ob ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ob ...

DIFFERENTIAL DISPLAYComparible RNAs were isolated from different treatments using my favorite method or any other methods you like and dissolved in RNA water. DNase treatment of 20ug total RNA 37oC 30 ...

Differential Display of Cotton TranscriptsSong P. Yamamoto E. and Allen R. (1995) Plant Mol. Biol. Rep. 13:174-181Plant MaterialsCotton ovules (Gossypium hirsutum cv. Coker 312) were collected 8 15 an ...

Chromatin IP MethodDay 11. Start 5 ml o/n cultures of strains to be testedDay 21. Inoculate 50 ml of the desired media with a volume of a saturated o/n culture and grow o/n with shaking at 200 rpm. Th ...

Human chromosome dimensionsChromosome # Relative length Centromere index 1 9.08 48.0 2 8.45 38.1 3 7.06 45.9 4 6.55 27.6 5 6.13 27.4 6 5.84 37.7 7 5.28 37.3 X 5.80 36.9 8 4.96 35.9 9 4.83 33.3 10 4.68 ...

The differential display protocol described here is based on the principle described by: Liang P. and A.B. Pardee : Differential display of eukaryotic messenger RNA by means of the polymerase chain re ...

All living organisms have thousands to tens of thousands of unique genes encoded in their genome of which only a small fraction perhaps 15% are expressed in any individual cell. Therefore it is the te ...

Optimal arbitrary primer length for Differential DisplayThe concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to ...

Optimal arbitrary primer length for Differential DisplayThe concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to ...

Reagents:ChIP sonication Buffer (1% Triton X-100 0.1% Deoxycholate 50 mM Tris 8.1 150 mM NaCl 5 mM EDTA):10 ml 10% Triton X-1001 ml 10% Deoxycholate5 ml 1 M Tris-Cl pH 8.11 ml 0.5 M EDTA3 ml 5 M NaCl ...

CHIP PROTOCOL FOR YEASTCHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTThis protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was writte ...

Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial and error. ...

Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial and error. ...

Chromatin IP (CHIP assay)This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. Gen & Dev 11 p83-93 and was obtained from Flick K. (The Scripps Research Ins ...

Chromatin IP (CHIP assay)This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. Gen & Dev 11 p83-93 and was obtained from Flick K. (The Scripps Research Ins ...

CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTCHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST2 Days Before--Start 5 mL overnight from single colony.1 Day Before--Subculture overnight ...