DNA制备

Construction and Characterization of Adenovirus VectorsP. Joel Ross and Robin J. Parks Adapted from Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). CSHL Press Cold Sp ...

Construction and Characterization of Adenovirus VectorsP. Joel Ross and Robin J. Parks Adapted from Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). CSHL Press Cold Sp ...

第二章 酵母双杂交筛选 1. 操作流程 诱饵基因转化筛库宿主菌Y190――含诱饵基因的Y190 保种――诱饵基因的 自激活检测――筛库(组织库或者小文库)――挑选鉴定阳性克隆――抽提阳 性克隆中PREY 质粒并在细菌中扩增――猎物(Prey)质粒与诱饵(Bait)质粒共 同转化Y190，验证其相互作用 2. 方 法 2. 1 诱饵基因转化筛库宿主菌Y190 1） 3 个1mm 克隆接种于3mlYP ...

山东博奥克生物科技有限公司分子生物学工具酶（Taq、Pfu、Hotstar Taq、MMLV、各种PCR Mix等）厂家直销，掀起低价风暴，绝对物超所值诚招代理！山东博奥克生物科技有限公司分子生物学工具酶（Taq、Pfu、Hotstar Taq、MMLV、各种PCR Mix等）厂家直销，掀起低价风暴，绝对物超所值诚招代理！ ...

第一章 基因克隆 1. 操作流程 收集基因信息——设计引物——PCR ——回收目的片段——与载体 连接，取得连接产物 —— 用感受态细胞做转化 ——接菌——阳性克隆鉴 定(酶切法或PCR)——质粒抽提——质粒测序——测序结果分析——克隆 信息输入数据库——质粒实物保存 2. 方法 2.1 设计引物 引物设计要求：引物长度为25bp-35bp.forward primer 和reverse prim ...

1、核酸抽提原理 简单地讲，核酸抽提包含样品的裂解和纯化两大步骤。裂解是使样品中的核酸游离在裂解体系中的过程，纯化则是使核酸与裂解体系中的其它成分，如蛋白质、盐及其它杂质彻底分离的过程。 经典的裂解液几乎都含有去污剂 (如 SDS、Triton X-100、NP-40、Tween 20 等) 和盐 (如Tris、EDTA、NaCl 等)。盐的作用，除了提供一个合适的裂解环境 (如 Tris)，还 ...

This is a question asked quite a bit most recently (late September 1995) in the newsgroup. Here are two methods and who submitted them. ...

来自加拿大多伦多大学，多伦多成瘾与心理卫生研究中心，以及瑞士，澳大利亚的研究者在最新一期的Nature Genetics发表研究成果，该研究文章在遗传学方面提出了新的问题，质疑DNA作为唯一遗传物质的学说。一直以来，经典的遗传学说认为DNA是遗传信息的载体，人类所有的信息都存在于这个小小的染色体上。从父母亲那遗传来的形状取决于DNA序列。文章作者Art Petronis教授否定了这一论断，他认为D ...

作分子生物学实验，核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆，通常要作双酶切。为了省时省事儿，我们常常想方设法把两个酶放在一起切。可是事情并非总那么称心如意――两个酶经常在一起闹别扭，不是反应温度不同啦，就是Buffer不同，有时候两个酶切位点连在一起，必须先切一个再切另一个，否则就切不动――因为有的酶除了识别位点之外还需要旁边留有几个碱基，如果正好被切掉了就出问题。于是有人就到处找 ...

This is a modification of the procedure in Short Protocols (1-411-45)1. Prepare a 50 ml liquid lysate:A. mix 2xlO8 E. coli cells with 100 ul phage (from one picked plaque in 500 ul SM) and100 ul lOmM ...

Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligonucleotide will ...

Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37℃ overnight on a roller.Transfer this overnight culture to a 1.7 ...

This protocol was written by Jean-Pierre Issabased on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity i ...

DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group catalyzed by DNA methyltransferase (DNMT) to the 5-c ...

FootprintingFootprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: hormone-receptor complexes that bind to their hormone response el ...

Contributed by Dr. A. GratchevSingle Nucleotide Primer Extension is a powerful method which can be used for the precise analysis of methylation in a certain position. The procedure is shown on the fig ...

1. DNA DNA template plasmid 5-20 ng 　　10x pfu DNA polymerase buffer 5.0 µl 　　25uM oligo 1 0.5 µl 　　25uM oligo 2 0.5 µl 　　10mM dNTP 1.0 µl 　　Pfu DNA polymerase (2.5 units) 1.0 µl 　　fill w/ddH2O to 50 µ ...

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The Erase-a-Base® System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA . The system is based on the procedure d ...

Mutagenesis is a fundamentally important DNA technology which seeks to change the base sequence of DNA and test its effect on gene or DNA function. The mutagenesis can be conducted in vivo (in studies ...