DNA重组

1) Grow 3-5 ml over-night E. coli cultures containing your plasmid2) Spin down 1.5 ml cells3) Resuspend in 300 µl Buffer P1 w. RNase A4) Add 300 µl Buffer P2.Mix by invertin ...

Stuff you need:TENS Buffer10 mM Tris-HCl pH 8.01 mM EDTA pH 8.00.1 N NaOH0.5% SDSTE pH 8.010 mM Tris-HCl pH 8.01 mM EDTA pH 8.095% EtOH precooled to -20 deg C70% EtOH at room temperature3 M Na Acetate ...

Objective:Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis seque ...

PREPARE SOLUTIONS 1. Glycerol mix (1 L):Weigh 25 grams (liquid weight) of glycerol and add dH2O to 1 Liter (Autoclave)2. Potassium mix (1 L):Mix 170 mL of 1M KH2PO4 (monobasic) 720 mL of 1M K2HPO4 (di ...

1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml m ...

锐博生物新的EdU荧光标记技术，能够方便、快速、准确的检测研究细胞的增殖、周期、凋亡、活性、分化、迁移及示踪。详细使用说明请见下面的PDF文档！C10310&C10312 Cell-Light TM EdU荧光显微镜检测试剂盒说明书.pdf需要订购相关产品请填好下面的合同和信息卡发到：sales-cd@ribobio.com！EdU&EU 订购信息卡.xlsRibobio 2010年购销合 ...

锐博生物新的EdU荧光标记技术，能够方便、快速、准确的检测研究细胞的增殖、周期、凋亡、活性、分化、迁移及示踪。详细使用说明请见下面的PDF文档！C10311 Cell-Light TM EdU 流式细胞仪 检测说明书.pdf需要订购相关产品请填好下面的合同和信息卡发到：sales-cd@ribobio.com！EdU&EU 订购信息卡.xlsRibobio 2010年购销合同.doc ...

mRNA-Seq_SamplePrep_1004898_D.pdf

Small RNA Sequencing Sample Preparation Guide.pdf

Preparing of cell extracts ...

DNA and RNA EXTRACTIONS A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol) ...

PlasmidsA DNA molecule can be amplified using the in vitro technique of PCR to obtain large number of identical molecules. Such pure DNA is needed for example to sequence it to use as a probe in South ...

PlasmidsA DNA molecule can be amplified using the in vitro technique of PCR to obtain large number of identical molecules. Such pure DNA is needed for example to sequence it to use as a probe in South ...

ProcedurePurify the PCR product. Before adding the overhangs it is very important to remove all the Proofreading DNA Polymerase (Pfu) by purifying the PCR product carefully (e.g. with a commercial PCR ...

After amplification with a proofreading polymerase place samples on ice and add 0.7-1 unit of Taq polymerase per tube directly into the PCR reaction tube. Mix well. Incubate at 72°C for 8-10 minutes. ...

This protocol uses Promega''s pGEM-T kit (#A3600).PCRFor TA cloning it is optimal if the PCR primers have G''s at the 5'' end as this will maximize the probability of Taq polymerase adding the termina ...

Here is some informal background information that will hopefully provide support and context if you want to use ET recombination. Perhaps needless to mention this information has not been refereed is ...

AbstractFunctional genomics require manipulation and modification of large fragments of the genome. Such manipulation has only recently become more efficient due to the discovery of different techniqu ...

A stab culture is made by inoculating bacteria into a vial containing LB agar with the appropriate antibiotic. After overnight incubation bacterial growth should be visible both in the puncture and on ...

Proteinase K digestion Mix DNA extraction buffer 98 μl ReagentB 2 μl ProteinaseK Mix fresh. 100 μl is enough for a small pea size chunk of tissue or one embryo Place small piece of tissue or embryo in ...