DNA重组

SolutionsPBSCell lysis buffer: 10 mM Tris pH8 100 mM NaCl 25 mM EDTA 0.5% (w/v) SDS 0.5 mg/mL proteinase KFixative: 1 volume glacial acetic acid + 3 volumes methanolTrizol reagent75% EtOH made with ...

The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however its high rates of DNA ...

Epigenetics is the study of heritable changes in gene expression. Chromatin immunoprecipitation (ChIP) and methylation status analysis of genes have been applied to the study of epigenetic modificatio ...

Make sure that there is enough dCTP and TTP (or dGTP and dATP) in the hot room Set up your PCR master mix. Remember that you need to do a reaction for dCTP and TTP for each sample 10xPCR Buffer 2.5u ...

Ancient DNA extraction from bones and teeth.pdf ...

Non_destructive_Biotechniques_2004art.pdf

Tissue preparation We used FFPE tissue blocks of Non-small cell lung cancer obtained from Pathology department of Kerman Medical University which were fixed from 2004 to 2006. Before outset of our exa ...

Non_destructive_Biotechniques_2004art.pdf

Meyerowitz et. al. (~1987ish) A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysacchar ...

Nuclei isolation modified from Hamilton Kunsch and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless ...

Meyerowitz et. al. (~1987ish) A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysacchar ...

Nuclei isolation modified from Hamilton Kunsch and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless ...

Use fresh or Lyophilize the leaf tissue. Grind about 5 g leaf tissue with mortar and pestle in liquid N2 transfer the powder into an 50 ml polypropylene tube (50 ml) and store at -20°C. Add 25 ml of ...

This protocol originated in Murray and Thompson (1980) and then was modified by Richard Jorgensen and finally published in Wagner et. al 1987.Start with about 10 grams fresh needles. Chop into short ...

CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents ...

Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq. N2 in a mortar. We normally use some alumina to crush hard tissue. Transfer the ground tissue to a eppendorf tube. Add 1 ml ...

Plant_Leaf_DNA_Extraction.pdf

Plant_Leaf_DNA_Extraction.pdf

Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar and pestle. Add 0.5 ml of TE buffer to the tube. Centrifuge at 500 g ...

Grow Rhizobium cells in 5 ml of YEMB at 200 rpm till the O.D (600 nm) reaches 0.6-0.8. Pellet the cells by centrifugation at 10000 rpm for 15 mins. Wash the pellet with TE buffer (10T/1E). Dissolve th ...