DNA重组

DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver NitrateSource: Contributed by Mohammad Reza Abbaszadegan et al.Abstract: Single strand conformational polymorphism (SSC ...

Electro-elution of nucleic acids from agarose and polyacrylamide gel slides.PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped ...

Electro-elution of nucleic acids from agarose and polyacrylamide gel slides.PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped ...

Recovering DNA from agarose gels Paul N. Hengen Ph.D. (July 14 1999) Introduction========Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.mol ...

Materials: TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)Frozen agarose gel piece containing the desired DNA fragmentSupplies: Micropipetter and tips Microcentrifuge and tubes Sp ...

Rapid elution of DNA from agarose gelsJames Movius Hahn Lab Last modified Sun Nov 1 1998A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restrict ...

Materials: TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)Frozen agarose gel piece containing the desired DNA fragmentSupplies: Micropipetter and tips Microcentrifuge and tubes Sp ...

PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKSCut 10-20X 10 um sections of formalin fixed paraffin samples into eppendorf tubes. Add 1 ml xylene mix incubate at 55 C for 15 mins. Release pressure spin d ...

1. Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55EC for 5 min or until the gel dissolves.3. ...

1. Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55EC for 5 min or until the gel dissolves.3. ...

DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is prefe ...

DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is prefe ...

DNA Extraction from Archival Formalin-fixed Paraffin-embedded Tissue SectionsAuthor: Shi et al.Source: Contributed by APostodocAbstract: Describes two methods of extracting DNA from archived paraffin- ...

DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONSThis is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologist ...

DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONSThis is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologist ...

Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq. N2 in a mortar. We normally use some alumina to crush hard tissue. Transfer the ground tissue to a eppendorf tube. Add 1 ml ...

Southern 杂交是分子生物学的经典实验方法。其基本原理是将待检测的DNA样品固定在固相载体上，与标记的核酸探针进行杂交，在与探针有同源序列的固相DNA的位置上显示出杂交信号。通过Southern杂交可以判断被检测的DNA样品中是否有与探针同源的片段以及该片段的长度。该项技术广泛被应用在遗传病检测、DNA指纹分析和PCR产物判断等研究中。但由于该技术的操作比较烦琐、费时，所以现在有一些其他的 ...

Screening Colonies by Hybridization with Radiolabeled ProbeLysis of Colonies and Binding of DNA to Nitrocellulose Filters Labeling of DNA-Probe with 32P Hybridization to Nitrocellulose Filters Contain ...

第一节 概 述 　　质粒具有稳定可靠和操作简便的优点。如果要克隆较小的DNA片段(＜10kb)且结构简单质粒要比其它任何载体都要好。在质粒载体上进行克隆从原理上说是很简单的，先用限制性内切酶切割质粒DNA和目的DNA片段 然后体外使两者相连接 再用所得到重组质粒转化细菌即可完成。但在实际工作中 如何区分插入有外源DNA的重组质粒和无插入而自身环化的载体分子是较为困难的。通过调整连接反应中外源DNA ...

第一节 概 述 　　质粒具有稳定可靠和操作简便的优点。如果要克隆较小的DNA片段(＜10kb)且结构简单质粒要比其它任何载体都要好。在质粒载体上进行克隆从原理上说是很简单的，先用限制性内切酶切割质粒DNA和目的DNA片段 然后体外使两者相连接 再用所得到重组质粒转化细菌即可完成。但在实际工作中 如何区分插入有外源DNA的重组质粒和无插入而自身环化的载体分子是较为困难的。通过调整连接反应中外源DNA ...