抗原设计

● PCR试剂和设备● 含有起始scFV基因的载体pHENl单链模板DNA（50ng/ul）● Geneclean试剂盒● Wlzard PCR纯化试剂盒● scFvJHL2XhoFOR引物：5'-GAG TCA TTC TCG TCT CGA GAC GGT GAC CAG GGT GCC-3'● scFvJH3XhoFOR引物： 5-GAG TCA TTC TCG TCT CGA ...

Purpose and backgroundsAbout nuclide...35-SDecay: (b-ray 0.167 MeV)Half life: 87.5 daysThick acrylic shield can block most of the emission. Because of the low energy it is impossible todetect spillage ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

PurposeMaterialsAntibody 7E3 2L sup grown in flasks frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 10 ...

PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid quantitative multiparameter analyses on singl ...

PurposeThis is a guideline for animal experiment. ProcedureAnimal Protocol An active animal protocol is needed for any experiment involving animals. If the experiment is not covered by the existing an ...

IntroductionGreen fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien 1996; Tsien 1998). The validation and inte ...

IntroductionRNA-protein interactions play important roles within the cell. Using a variation of the widely-used chromatin immunoprecipitation (ChIP) assay the potential association of cellular RNAs an ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Conjugation of carrier (BSA) to peptide1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate pH 7.0.2. Dissolve MBS-SMCC @ 25mg/ml in NNdimethylformamide.3. Mix 20ml SMCC and 200ml BSA (500mg:4000mg or 1: ...

The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recomb ...

There are many different types of ELISAs which can detect the presence of protein in serum or supernatent. One of the most common types of ELISA is the so-called "sandwich ELISA." It is termed this be ...

You will need the following solutions: PBS (Phosphate-Buffered Saline Ca/Mg free) :- Solute1x10xNaCl8g80gKCl0.2g2gNa2PO4 (anhydrous)1.15g11.5gKH2PO4 (anhydrous)0.2g2gN.B.: Na2HPO4.2H2O can also be use ...

Sensitize a 96-well microtiter plate with purified antigen. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 1 ...

Immunoassays are a powerful technique for detecting and measuring antigens and antibodies. Immunoassays can be classified three ways based on the steps involved: antibody capture antigen capture two ...

OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) ( ...

This protocol can be used for detection of gangliosides.Spicific antibodies to gangliosides are added to cells and allowed to bind to the cell surface. The nonbound antibody is washed away and an enzy ...

Reagents1. DMEM:10% FBS2. DMEM:10% FBS:1% HEPES (DFH)3. 10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g Potassium dibasic phosphateDissolve in 900 ml di-H2O. Ch ...

PBSPBS:0.1% BSA:0.05% Tween 20 (PBT)PBS:2% BSA10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g Potassium dibasic phosphateDissolve in 900 ml di-H2O. Check pH and adjust to ...