其他模型

High Efficency Yeast Plasmid Rescue(by Trey Powers Fields Lab) Grow yeast overnight.Spin down 1.5 ml of culture in microfuge tubes.DecantAdd 250 µl of Qiagen buffer P1 to each tubeAdd about 250 µl of ...

Yeast Prep for FACS 7(13):4335-4346; 1988.1. Spin down 1E7 cells in a microfuge tube for 1 minute.2. Resuspend pellet in 1 ml of 70% EtOH. Fix for at least 60 minutes at room temperature (or up to sev ...

Yeast Cell LysatesModified from the Rine LabGrow cells to 0.3 to 0.5 O.D.(600) (~1E7 cells/ml). Use about 1.0 to 5.0 OD of cells per lysate; if your cells are at 0.5 OD per ml use 2 ml to give you 1.0 ...

Preparation of Yeast Protein Extracts ...

Yeast Protein Isolation "Yaffe-Schatz"David AmbergAdapted from Yaffe M.P. and Schatz G. PNAS 1984 81 4819-4823.Grow 25mls yeast cells to 5x10E6. Sequentially spin down cells in a 15 ml polypro tube. W ...

Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J. Leatherwood Ptashne lab)last modified Mon Aug 27 2001Cell Growth3 liters of cells are grown in YPD (3% glucose) to A600 of 3-5. Antifoam ...

Yeast RNA Miniprepgrow cells to mid-log phase (10-20 ml) (or take samples from time courses 5-25 ml) spin cells discard supernatant wash cells once with 1 ml cold RNA buffer freeze pellet in dry ice ( ...

Pol III whole cell yeast extract (small scale)Mike Schultz Pavel Aprikian Steve Hahnlast modified6/28/99Grow 2 liters yeast in YPD (with 3% glucose) to an A600 of 1.5 to 2.0 (inoculate ~1.5 ml satura ...

Curing strains of endogenous 2-micron plasmidIn order to create a circle-zero derivative from a Cir+ strain the method of Rose and Broach (1990) is used. A 2-micron-based plasmid YEp351-GAL-FLP1 * tha ...

Rapid Yeast Protein Prep for SDS PAGE and WesternHorvath and Riezman Yeast 1994; Gottschling LabSample Buffer: 10 ml0.06M Tris-HCl pH 6.80.6 ml 1M Tris 6.810% (v/v) glycerol2 ml 50% glycerol2% (w/v ...

EMS Mutagenesis of Yeast Linda Hoskins Hahn Lab Last modified10/16/981. Grow a 50 ml O/N YPD yeast culture at 30 degrees C.2. Determine cell density and transfer an amount of culture corresponding to ...

ß - GAL Filter Assaystreak yeasts to single colony replica plate onto NC-filter (leave 10 sec.) use tweezers to lift filter and slowly submerge in liquid Nitrogen for 1 min place on back side of a pet ...

Making library DNA from the DNA we send youThe original library was distributed as 18 individual pools. After 8/98 there is a new library that is distributed as 10 pools. You will be sent about a mi ...

mTn-4xHA/lacZ TRTn3 terminal inverted repeatsHAHemagglutinin (HA) epitope loxRlox site target for Cre recombinaselacZ5'-truncated lacZ gene encoding beta-galactosidaseURA3URA3 gene from S. cerevisiaet ...

Mutagenizing a yeast gene with a mini-transposonFirst clone gene into vector pHSS6. Then:Day 1: Transform plasmid into bacteria expressing transposase. Day 2: Mate transformants to bacteria carrying m ...

Making library DNA from the DNA we send youThe library is distributed as individual pools in the form of DNA. You will be sent about a microgram of each pool available. Transform a suitable amount in ...

mTn-3xHA/lacZ TR Tn3 terminal inverted repeatsXaFactor Xa cleavage recognition siteloxRlox site target for Cre recombinaselacZ5'-truncated lacZ gene encoding beta-galactosidaseURA3URA3 gene from S. ce ...

mTn-lacZ/LEU2 TRTn3 terminal inverted repeatslacZ5'-truncated lacZ gene encoding beta-galactosidaseLEU2LEU2 gene from S. cerevisiaeampEncodes beta-lactamaseloxPlox site target for Cre recombinaseUses: ...

Quantitative Mating AssayGuthrie and Fink Guide to Yeast Genetics and Molecular Biology 19911. Grow a culture of the strain whose mating efficiency is to be determined to a density of about 1E7 cells/ ...

Replication timing by comparative hybridizationM. K. RaghuramanReference: Friedman K. L. M. K. Raghuraman W. L. Fangman and B. J. Brewer (1995) Analysis of the temporal program of replication initiati ...