结构与功能

Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of pl ...

Calcium (Ca2+) is a key component of the signalling network by which plant cells respond to developmental and environmental signals. A change in guard cell cytosolic free Ca2+(cyt) is an early event in the response of stomata to both opening and closing stimuli, and cyclic nucleotide-mediated Ca ...

Phosphoproteomics is a fast-growing field that aims at characterizing phosphorylated proteins in a cell or a tissue at a given time. Phosphorylation of proteins is an important regulatory mechanism in many cellular processes. Gel-free phosphoproteome technique involving enric ...

To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integration in Arabidopsis thaliana, designated CORrelation NETworks (acronym CORNET), allowing browsing of microarray data, construction of ...

Transcriptome coexpression analysis is an excellent tool for predicting the physiological functions of genes. It is based on the “guilt-by-association” principle. Generally, genes involved in certain metabolic processes are coordinately regulated. In other words, coexpres ...

The recent development of various deep sequencing techniques has led to the most powerful transcript profiling method available to date, RNA sequencing or RNA-Seq. Besides the identification of new genes and new splice variants of known genes, RNA-Seq allows to compare the whole transcri ...

cDNA-AFLP is a commonly used, robust, and reproducible tool for genome-wide expression analysis in any species, without requirement of prior sequence knowledge. Quantitative expression data are generated by gel-based visualization of cDNA-AFLP fingerprints obtained by selec ...

Plants accumulate an overwhelming variety of secondary metabolites that play important roles in defense and interaction of the plant with its environment. To investigate the dynamics of plant secondary metabolism, large-scale untargeted metabolite profiling (metabolomi ...

Metabolomics based on nuclear magnetic resonance (NMR) can be used to monitor the metabolite response of plants to jasmonic acid. Metabolomics experiments consist of three important steps: sample preparation, NMR analysis, and data mining. In sample preparation, a very critical fact ...

The endogenous concentration of N-jasmonoyl-l-isoleucine (JA-Ile) is regulated by the balance between biosynthesis and deactivation and controls plant developmental processes and stress responses. Therefore, profiling of its precursors and metabolites is required to un ...

The Nicotiana tabacum lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory. Here, we describe different techniques by which lectin production can ...

Jasmonic acid (JA) is a plant hormone that mediates a wide variety of plant developmental processes and defense responses. One of the major roles of JA is the versatile enhancement of the production of secondary metabolites that function as second messengers in plant defense responses. Rece ...

The sequence information generated through genome and transcriptome analysis from plant tissues has reached unprecedented sizes. Sequence homology-based annotations may provide hints for the possible function and roles of particular plant genes, but the functional annota ...

The electrophoretic mobility shift assay based on nondenaturing polyacrylamide gel electrophoresis is a simple, rapid, and sensitive method for the study of the interaction of transcription factors with DNA in vitro. It relies on a change in the electrophoretic mobility of a DNA fragment ...

Protein–protein interactions play important roles in many cellular processes, including the regulation of phytohormone signaling pathways. Identification of interacting partners of key proteins involved in the cellular signaling control can provide potentially unex ...

The jasmonate (JA) ZIM-domain (JAZ) proteins of Arabidopsis thaliana repress JA signaling and negatively regulate the JA responses. Recently, JAZ proteins have been found to inhibit the transcriptional function of several transcription factors, among which the basic helix-loop- ...

Protein–protein interaction studies are crucial to unravel how jasmonate (JA) signals are transduced. Among the different techniques available, yeast two-hybrid (Y2H) is commonly used within the JA research community to identify proteins belonging to the core JA signaling module. ...

Pull-down assays are key tools to test specific protein–protein interactions and have been particularly fruitful in jasmonate signaling research. Here, we describe a standard protocol in which a matrix-bound “bait” protein, expressed in Escherichia coli, pulls down a “prey” protein t ...

Jasmonic acid (JA) is activated for signaling by its conjugation to isoleucine (Ile) through an amide linkage. The Arabidopsis thaliana JASMONIC ACID RESISTANT1 (JAR1) enzyme carries out this Mg-ATP-dependent reaction in two steps, adenylation of the free carboxyl of JA, followed by cond ...

To determine the location of specific molecules within tissues or cells, immunological techniques are frequently used. However, immunolocalization of small molecules, such as jasmonic acid (JA) and its bioactive amino acid conjugate, JA-isoleucine, requires proper fixation and ...