疾病及处理

This short overview attempts to highlight the current state of the art relevant to bacterial protein toxins. In particular we outline the major achievements in this field during the past decade and briefly describe some significant hallmarks of toxinological research since the advent of ...

The use of cell cultures for the laboratory diagnosis of Chlamydia trachomatis infections was popularized during the 1970s and 80s (1–4). The techniques required live organisms and were restricted to specialized laboratories. During the 1980s the detection of chlamydia-specific a ...

Hepatitis B virus (HBV) is a member of the Hepadnaviridae family and has a (3200-bp) partially double-stranded circular DNA genome (1). This virus causes subclinical, acute self-limited, chronic, and fulminant hepatic infections. Chronic HBV infection is particularly important bec ...

The isolation of a retrovirus from peripheral blood lymphocytes/monocytes can be a difficult task, requiring the fulfillment of three essential parameters. First, this viral agent must infect such cells in vivo. Second, these circulating cells should harbor wild-type proviruses. Fi ...

Mycoplasmas are the smallest prokaryotes capable of self-replication. They belong to the class Mollicutes (meaning soft-skin) and have evolved regressively, by genome reduction, from Gram-positive bacterial ancestors, namely certain clostridia (1). The taxonomy of the class Mo ...

The three major causes of genital ulcer disease (GUD) are herpes simplex virus (HSV), Treponema pallidum, and Haemophilus ducreyi. Although techniques exist for the laboratory diagnosis of all three organisms, constraints of cost, availability of equipment and expertise, and the lack of ...

Genital ulcers are typically caused by one of three organisms, Haemophilus ducreyi, Treponema pallidum, or herpes simplex virus, which cause chancroid, syphilis, and genital herpes, respectively. Although traditionally these diseases have been differentiated by their clinic ...

Sexually transmitted diseases have afflicted humankind for millennia, based on references to apparent gonorrhea or nongonococcal urethritis in the Old Testament (Leviticus). For most of history there has been no means of specific diagnosis, and clinical diagnosis of syndromes was f ...

Molecular techniques have gradually shifted the paradigm in the laboratory diagnosis of sexually transmitted infections from biological to molecular amplification. It is now possible to combine the sensitivity and specificity of culture with all the convenience of nonculture ...

Semen is the major vehicle for the sexual transmission of HIV-1. The ability to isolate infectious HIV from the semen and to quantitate viral burden in the form of cell-free or cell-associated HIV-1 RNA in semen are important for epidemiologic and public health aspects of the epidemic. Earlier stud ...

The ability to measure accurately viral RNA in the plasma (1–3) and intracellular (4–7) compartments of HIV-1-infected persons has led to a dramatic improvement in our understanding of the natural history of HIV-1/AIDS. A number of recent studies have convincingly demonstrated that high le ...

The papillomaviruses form a nonenveloped virion with an icosahedral capsid structure and contain a double-stranded circular DNA genome of 7800–7900 bp. The HPV genome is organized into three major regions; two protein-coding regions (1) early and (2) late-expressing genes, and (3) a nonco ...

Strain identification of Chlamydia trachomatis has historically been accomplished using serotyping as a phenotypic marker to differentiate chlamydial isolates (1). The target for serotyping is the major outer membrane protein (MOMP) which is the most antigenically diverse and ...

Neisseria gonorrhoeae, first described by Neisser in 1879, is a Gram-negative, nonmotile, nonspore-forming diplococcus, belonging to the family Neisseriaceae. It is the etiologic agent of gonorrhea. The other pathogenic species is Neisseria meningitidis, to which N. gonorrhoeae ...

Auxotyping (growth of cells on chemically defined media) and serotyping (reaction of cells with a defined set of monoclonal antibodies ) are the traditional and most widely used methods for the classification of Neisseria gonorrhoeae isolates. As an example, Knapp et al. (1) used the combina ...

Most individuals can be diagnosed as being infected with HIV-1 with an enzyme immunoassay (EIA), which detects antibodies to the virus. Repeatedly positive results must be confirmed, usually by Western blot. These assays have been improved since their introduction in 1985. When used toget ...

An improved Alu-long terminal repeat (LTR) polymerase chain reaction (PCR) assay is described for the quantification of integrated HIV-1 DNA in infected cells. The method includes generation of an infected cell line containing numerous randomly distributed HIV-1 integrated DNA for t ...

Integration is described as a key step in viral replication of all retroviruses. A sensitive and quantitative measure of an integrated molecule is a good way to examine the importance of the integration step and to evaluate efficiency of retroviral vectors for gene transfer or anti-integrase ...

The detection of the HIV-1 provirus that can integrate into a host cell nucleus and remain latent for years is problematic. The threshold of in situ hybridization, which is about 10 copies per cell, is too high to detect one integrated copy of the provirus. Although polymerase chain reaction (PCR) can de ...

In this chapter we describe a method for the detection of human T-cell leukemia virus type 1 (HTLV-1) genes in cytologic smears by polymerase chain reaction (PCR). First, already-stained and covered slides should be immersed in xylene for removal of cover slips. After passage through a descending ...