植物生理

In this chapter, we review the main organogenesis features and associated regulation processes of the moss Physcomitrella patens (P. patens), the model plant for the Bryophytes. We highlight how the study of this descendant of the earliest plant species that colonized earth, brings useful k ...

Cell-based computational modeling and simulation are becoming invaluable tools in analyzing plant �development. In a cell-based simulation model, the inputs are behaviors and dynamics of individual cells and the rules describe responses to signals from adjacent cells. The outpu ...

Ectocarpus siliculosus is a small filamentous alga that has recently emerged as the new model for fundamental research on brown algae. Here, we describe the basic culture protocols for propagating and collecting E. siliculosus material that can then be used in all types of molecular biology, b ...

Proteins are distributed in different cellular compartments. Our group studies the role of non-coding RNAs and associated RNPs in the development and stress response in legumes. Ribonucleoproteins (RNPs) are RNA-protein complexes that play different roles in many cellular proce ...

In the past decade, hundreds of non-coding RNAs (small and long RNAs) have been identified as crucial elements in developmental processes and stress response in plants. Among small RNAs, the microRNAs or miRNAs control levels of specific mRNA by inhibiting translation or reducing the stabi ...

Compound tomato leaves are composed of multiple leaflets that are generated gradually during leaf development, and each resembles a simple leaf. The elaboration of a compound leaf form requires the maintenance of transient organogenic activity at the leaf margin. The developmental w ...

Multiple ribosomes assemble onto an individual mRNA to form a polyribosome (polysome) complex. The epitope tagging of specific ribosomal proteins can enable the immunopurification of polysomes from crude cell extracts derived from cryopreserved tissue samples. Through expr ...

An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hor ...

The cellular level processes cell division and cell expansion form a crucial level linking regulatory processes at the molecular level to whole plant growth rates and organ size and shape. With the rapid progress in molecular profiling, quantification of cellular activities becomes in ...

Shoot apical meristems (SAMs) of higher plants harbor a set of stem-cells and provide cells for the development of all the above-ground biomass of plants. Most of the important pattern formation events such as maintenance of stem-cell identity, specification and differentiation of leaf/ ...

The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expression during plant development. The sites of IAA biosynthesis and its polar transport (PAT) routes determine auxin accumulation and dis ...

The analysis of gene expression at transcript and at protein level is of outstanding importance in plant developmental biology. Proteins can be localized with subcellular resolution by immunolocalization using specific antibodies or generating reporter lines carrying the sp ...

Developmental biologists require methods for marking cell lineages as they arise in living tissues. Traditionally, lineages have been traced in fixed tissues but these observations are difficult to verify. We present a method by which a progenitor cell and all of its lineage become marked by a ...

Development of the root system is essential for proper plant growth and development. Extension of the root system is achieved by the continuous establishment of new meristems in existing parental root tissues, which leads to the development of lateral roots. This process of lateral root org ...

Root systems are complex structures key to plant health. The three-dimensional distribution of the root system, known as the root architecture, is important for optimal uptake of water and nutrients, as well as anchorage in the soil. Despite the importance of root systems, little is known about t ...

Adventitious root formation, the development of roots on non-root tissue (e.g. leaves, hypocotyls and stems) is a critical step during micropropagation. Although root induction treatments are routinely used for a large number of species micropropagated in vitro as well as for in vivo cutt ...

Gene flow is one of the major concerns associated with the release of transgenic plants into the environment. Unrestricted gene flow can results in super weeds, reduction in species fitness and genetic diversity, and contamination of traditional plants and foods. Thus, it is important and al ...

An optimized vip3A gene, designated as vip3undefined was chemically synthesized and a thi1 gene chloroplast transit peptide coding sequence was attached to its 5′ end to produce the tvip3undefined. vip3undefined and tvip3undefined genes were transformed into Gossypium hirsutum cv. Zhongmiansuo35 mediated by Agrobac ...

The soil-borne fungal pathogen Verticillium dahliae Kleb causes Verticillium wilt in a wide range of crops including cotton (Gossypium hirsutum). To date, most upland cotton varieties are susceptible to V. dahliae and the breeding for cotton varieties with the resistance to Verticill ...

Cotton is an important world economic crop plant. It is considered that cotton is recalcitrant to in vitro proliferation. Somatic embryogenesis and plant regeneration has been successful by using hypocotyl, whereas it is highly genotype dependent. Here, a genotype-independent cott ...