测序实验技术

在分子生物学研究中，DNA的序列分析是进一步研究和改造目的基因的基础。目前用于测序的技术主要有Sanger等（1977）发明的双脱氧链末端终止法和Maxam和 Gilbert（1977）发明的化学降解法。这二种方法在原理上差异很大，但都是根据核苷酸在某一固定的点开始，随机在某一个特定的碱基处终止，产生A，T，C，G四组不同长度的一系列核苷酸，然后在尿素变性的PAGE胶上电泳进行检测，从 ...

Introduction For over 25 years Applied Biosystems has been a pioneer in the field of genetic analysis by offering systems to address the expansion of genetic analysis applications and the evolving ne ...

ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases adenine guanine cytosine 5-methyl cytosine and thymin ...

ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases adenine guanine cytosine 5-methyl cytosine and thymin ...

technology: illumina sequencing technology This breakthrough platform is based on massively parallel sequencing of millions of fragments using our proprietary reversible terminator-based sequencing ch ...

One Fragment = One Bead = One ReadThe complete sequencing workflow of the Genome Sequencer FLX System comprises four main steps leading from purified DNA to analyzed results. These basic steps include ...

454 Life Sciences is a biotechnology company based in Branford Connecticut specializing in high-throughput DNA sequencing using a novel massively parallel sequencing-by-synthesis approach. 454 has exp ...

一、测序的基本原理： 测序的基本原理是Sanger末端终止法，在酶的作用下将原本是一条长达数百碱基的片断扩增成数百条片断，彼此间相差一个碱基。用电泳分离这些片断后，再通过测序仪将这些信号转变成峰图，从而最终形成客户所看到的测序序列和测序的峰图。 目前通用的扩增方式是通过PCR的方式进行的起源；其原理简述如下：PCR类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物。PCR由变 ...

DNA的复制需要：DNA聚合酶，单链DNA模板，带有3'-OH末端的单链寡核苷酸引物，4种dNTP（dATP、dGTP、dTTP和dCTP）。聚合酶用模板作指导，不断地将dNTP加到引物的3'-OH末端，使引物延伸，合成出新的互补DNA链。如果加入一种特殊核苷酸，双脱氧核苷三磷酸（ddNTP），因它在脱氧核糖的3’位置缺少一个羟基，故不能同后续的dNTP形成磷酸二酯键。如，存在ddCT ...