化学生物学实验技术

AbstractFour methods (an enzyme activity assay western blotting RT-PCR and northern hybridization) to detect the enzyme D-amino-acid oxidase are described. Introduction ...

AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helicobacter pylori by three diffirent methods. Nuclear magne ...

AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the ...

Pectinases are actually a mixture of enzymes which along with others such as cellulase are widely used in the fruit juice industry where they are widely used to help extract clarify and modify fruit j ...

This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells of these tomatoes have been genetically modified to ...

Browning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases. These enzymes are released by the broken cells and they catalyse the reac ...

In certain fruits such as pineapples and mangoes the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soften the fruit tissues as the fruit ripens making it ev ...

常用生化试剂货号品 名规 格价 格(元)备 注B1005Acridine Orange1/2 ZnCI2 (吖啶橙)5g/10g190/320Sigma分装B1006Acrylamide (电泳级丙烯酰胺)100g/500g120/500Sigma分装B1007AcrylamideUltra Pure 丙烯酰胺(超纯)100g/200g140/260Amresco分 ...

PurposeThe protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD ...

Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here we try to enlighten whether anesthesia with fentanyl/fluniasone and midaz ...

Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence bacteriocin production and other activities. These si ...

An important part of characterizing any protein molecule is to determine its size and shape. Sedimentation and gel filtration are hydrodynamic techniques that can be used for this medium resolution st ...

MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3′ untranslated region ...

Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competi ...

In the polymerase chain reaction (PCR) a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond to those on synthetic oligonucleotide primers whic ...

1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture.2. Grow larger culture (100x volume of starter culture) using the overnight culture as a seeding culture. The culture is gr ...

1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture.2. Grow larger culture using the overnight culture as a seeding culture. The culture is grown in LB-Amp medium. Aerate wel ...

Prepare the template by linearizing 25ug plasmid DNA at the 3'' end of the insert. Phenol / chloroform extract ethanol / NaCl precipitate and resuspend in 25ul DDW Transcription Reaction: Reagentamt / ...

在生物大分子纯化分析特别是蛋白质纯化分析中，色谱是非常重要而且常用的一种技术。 一、凝胶过滤　　凝胶过滤又叫分子筛色谱，其原因是凝胶具有网状结构，小分子物质能进入其内部，而大分子物质却被排除在外部。当一混合溶液通过凝胶过滤色谱柱时，溶液中的物质就按不同分子量筛分开了。 二、离子交换色谱　　离子交换色谱是在以离子交换剂为固定相，液体为流动相的系统中进行的。离子交换剂是由基质、电荷基团和反 ...

By: Christopher P. Toseland1 2 Martin R. Webb1 Affiliation(s): (1)MRC National Institute for Medical Research London UK(2)Institut für Zelluläre Physiologie Physiologisches Institut Ludwig Maximili ...