• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购

        化学生物学实验技术

        丁香实验推荐阅读
        Data Standards and Controlled Vocabularies for Proteomics

        Proteomics data can be diverse and complex, and are typically produced on a large scale. To allow sharing and centralized storage and dissemination of such results, the Human Proteome Organization (HUPO) Proteomics Standards Initiative (PSI) has created a set of community standards for t ...

        丁香实验推荐阅读
        Free-Flow Electrophoresis of the Human Urinary Proteome

        Prefractionation of complex protein samples prior to mass spectrometry provides a method for the isolation of low-abundance proteins into specific fractions thereby enabling their identification. Free-flow electrophoresis in the isoelectric focusing mode (IEF-FFE) pre ...

        丁香实验推荐阅读
        Toward a Full Characterization of the Human 20S Proteasome Subunits and Their Isoforms by a Combination of Proteomic Approaches

        The 20S proteasome is a multicatalytic protein complex, present in all eukaryotic cells, that plays a major role in intracellular protein degradation. In mammalian cells, this symmetrical cylindrical complex is composed of two copies each of seven different α and β subunits arranged into f ...

        丁香实验推荐阅读
        Exosomes

        Exosomes are small natural membrane vesicles released by a wide variety of cell types into the extracellular compartment by exocytosis. The biological functions of exosomes are only slowly unveiled, but it is clear that they serve to remove unnecessary cellular proteins (e.g., during ret ...

        丁香实验推荐阅读
        Identification and Characterization of N-Glycosylated Proteins Using Proteomics

        Glycoproteins constitute a large fraction of the proteome. The fundamental role of protein glycosylation in cellular development, growth, and differentiation, tissue development, and in host–pathogen interactions is by now widely accepted. Proteome–wide characterizati ...

        丁香实验推荐阅读
        Protein Processing Characterized by a Gel-Free Proteomics Approach

        We describe a method for the specific isolation of representative N-terminal peptides of proteins and their proteolytic fragments. Their isolation is based on a gel-free, peptidecentric proteomics approach using the principle of diagonal chromatography. We will indicate that the ...

        丁香实验推荐阅读
        Nondenaturing Mass Spectrometry to Study Noncovalent Protein/Protein and Protein/Ligand Complexes: Technical Aspects and Application to the Determinat

        In the present chapter we detail how mass spectrometry (MS) can be used to characterize noncovalent complexes, especially multimeric proteins and protein/ligand complexes. This original application of MS, also called “supramolecular MS” or “nondenaturing MS,” appeared in the early ...

        丁香实验推荐阅读
        Production of Protein Arrays by Cell-Free Systems

        Protein arrays make possible the functional screening of large numbers of immobilized proteins in parallel. To facilitate the supply of proteins and to avoid their deterioration on storage, we describe our protein in situ array (PISA) method for production of protein arrays in a single step d ...

        丁香实验推荐阅读
        Selection of Recombinant Antibodies by Eukaryotic Ribosome Display

        Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody–ribosome–mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by RT-P ...

        丁香实验推荐阅读
        Mapping of Signaling Pathways by Functional Interaction Proteomics

        Signaling pathways transduce extracellular stimuli from the membrane to the nucleus. Constitutive and thus inappropriate stimulation of these kinase cascades is associated with and observed in a majority of tumors. The transduction of signals in these pathways is achieved through ...

        丁香实验推荐阅读
        Native Fractionation: Isolation of Native Membrane-Bound Protein Complexes from Porcine Rod Outer Segments Using Isopycnic Density Gradient Centrifuga

        Networks of interacting protein control physiological processes in all living cells. Considerable effort has recently been invested in understanding protein interactions under normal and diseased conditions. One approach to elucidate the composition of protein complexes ...

        丁香实验推荐阅读
        Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons

        In this chapter, methods for designing metal ion sensors using fluorophore- and quencher-labeled DNAzymes are discussed. In contrast to the classical molecular beacon method based on binding, the methods described here utilize catalytic cleavage to release the fluorophore for det ...

        丁香实验推荐阅读
        Ribozymes: An Introduction

        One of the more important new insights in the field of molecular biology in the past 15 years was the discovery that RNA molecules, once thought to be primarily passive carriers of genetic information, can carry out some functions previously ascribed to proteins. Some RNAs are capable of acting as en ...

        丁香实验推荐阅读
        Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences

        The hairpin ribozyme has been developed and designed to target and cleave efficiently heterologous RNA sequences in a trans-reaction (1–3). Heterologous RNA cleavage has been carried out, targeting rules defined, systems identified, and very effective in vivo downregulation of gene ...

        丁香实验推荐阅读
        Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis

        The development of capillary electrophoresis (CE) technology has been rapid over the past three years for application to the analytical separation in a variety of biopoly-mers such as proteins, polysaccharides, and DNA (1–3). CE offers high throughput and high resolution, automatic ope ...

        丁香实验推荐阅读
        Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM)

        In comparison to the sequencing of other biological macromolecules such as nucleic acids or proteins, the sequencing of oligosaccharides (glycans) of glycoproteins generally requires specialized expertise and facilities Whereas techniques such as nuclear magnetic reson ...

        丁香实验推荐阅读
        Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays

        Recent advances in the mechanisms of regulation of leukotriene and prostaglandin biosynthesis, as well as their mechanisms of action, have generated a renewed interest in this field. One can cite the cloning of enzymes involved in the lipoxygenase (LOX) and cyclo-oxygenase (COX or PGHS) pat ...

        丁香实验推荐阅读
        Preparation of CollagenGlycosaminoglycan Copolymers for Tissue Regeneration

        Certain analogs of the extracellular matrix (ECM) have been shown to possess surprising morphogenetic activity during healing of lesions in various anatomical sites. This chapter describes methods for synthesis of the two ECM analogs that have been studied most extensively. The read ...

        丁香实验推荐阅读
        Analysis of Cannabis by Supercritical Fluid Chromatography with Ultraviolet Detection

        Cannabis sativa L. and its products comprise a significant and important part of the forensic drug laboratory’s case load. Two principle types of analyses are required for the analysis of Cannabis, namely, identification of the material, since it is a substance controlled in the United Kingdom ...

        丁香实验推荐阅读
        Analytical Methods for Optimization and Quality Control of Combinatorial Synthesis

        Rapid lead discovery in the drug development process relies on highthroughput screening of diverse compounds against protein targets. These compounds come from traditional organic synthesis, natural products isolation, and combinatorial synthesis (1). For compounds from c ...

        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序