化学生物学实验技术

Microfabric is the three-dimensional spatial arrangement of sediment grains and pore space in marine sediments. In addition to the mineral particles, consideration of the fluid-filled voids is essential for understanding sedimentological properties, such as permeability. T ...

A 28-year-old man was transferred to our hospital and underwent surgery for resection of an aortic graft infected with Klebsiella pneumoniae. Antimicrobial therapy consisted of amikacin, cefazolin, chloramphenicol, sulfamethoxazole, and trimethoprim. A request for amikacin ...

Until recently, it was difficult to explain why an identical drug dosage may exert a toxic effect in one patient and a therapeutic, or no, response in another patient. It has now been demonstrated that the concentration of drug at the tissue receptor site is the most important parameter for adjusting dr ...

High performance liquid chromatography (HPLC) is one of the most rapidly growing and potentially largest branches of analytical chemistry. Although further advances in HPLC are to be expected, current methodology is already far enough advanced to insure its use in the clinical laborat ...

The polyamines spermidine and spermine and their diamine precursor, putrescine , have been the subject of intense study relative to their potential as tumor markers during the past decade (1). These compounds have been implicated in numerous biochemical reactions and have been clearly a ...

The presence of increased levels of various organic acids in physiological fluids such as serum, plasma, and urine has been correlated with a variety of diseases (1). Although some are rare, others such as lactic acidosis and hyperoxaluria are more widespread (2, 3). The estimation of organic acids ...

Historically the term porphyria has been used since it was coined in 1871 to describe a purple colored material extracted from pathological feces (1). The first case of porphyria was reported in 1874, (2, 3), but until the 1930 Nobel Prize winning work of Hans Fischer on the synthesis of protoporphyrin, ...

Because of the complexity of cellular material and body fluids, it is seldom possible to analyze a natural product directly. Qualitative and quantitative analyses must often be preceded by some purification step that separates the molecular species being examined from interfering ma ...

The amino acids tyrosine and tryptophan are precursors for a number of important physiological compounds. The catecholamines, which are metabolites of tyrosine, serve as neurotransmitters in the central and peripheral nervous systems. Serotonin, a major metabolite of tryptoph ...

Rapid identification and quantitation of drugs in biofluids are helpful to the physician in managing patients with suspected drug intoxication. Higgins and O’Brien (1) noted that prior to 1960, drug overdoses usually consisted of a single drug. However, three years later they observed that ...

In recent years, most large hospitals have observed a marked increase in the admission of patients suffering from drug overdose. Overdose of narcotic drugs, such as the opiates, represent less of a problem on a day-to-day basis than do overdoses of prescribed drugs, such as sedatives and hypnotics. ...

This article reviews the basic principles of high performance liquid chromatography (HPLC). The introductory section provides an overview of the HPLC technique, placing it in historical context and discussing the elementary facts of the separation mechanism. The next section disc ...

Cryopreservation is the ultra-low temperature storage (usually in liquid nitrogen at ca. -135 to -196�C) of living cells, tissues and organs capable of resuming normal functions after retrieval from a cryobank. This chapter explains the basic principles of cryopreservation with resp ...

Morphological examination of calcified structures, by either light microscopy (LM) or electron microscopy (EM) often requires a decalcification (decal) step after fixation. Further processing steps must await the completion of the decal process. The time required for full decal wi ...

The authors’ research focuses on the structural basis of synaptic function. Knowing the dimensions and connectivity of presynaptic and postsynaptic elements is necessary for understanding the mechanisms of synaptic transmission in the central nervous system. To this end, electr ...

Preparation of botanical specimens for electron microscopy (EM) is hampered by several physical factors unique to plant cells. Impediments to penetration and action of various reagents in the tissue preparation process include the vacuole, plastids, cell wall (CW), and intercellul ...

Microwave (MW) technology has found a permanent niche in the world of surgical pathology, biomedical research, and allied disciplines, for a few simple reasons: It can reduce the processing times for tissue samples destined for evaluation by light, scanning, or electron microscopy (EM); it is ...

Chemical (chem) fixatives and conventional fixation times, used for standardized preservation of tissues, can result in serious alterations to morphology, as a consequence of solubilization and conformational changes of proteins and lipids (Login and Dvorak, 1985). These untow ...

Acetylcholinesterase (AChE) is the modulating enzyme of cholinergic systems. Study of its morphological distribution and its pathophysiological disposition following disease or exposure to anticholinesterase compounds such as pesticides, organophosphates, and ch ...

关键词： 免疫球蛋白 蛛网膜下腔 　　免疫球蛋白(immunoglobulin,Ig)鞘内合成理论，近年来成为神经免疫学领域的一个热点问题。关于鞘内Ig的来源，说法不一，但各种观点的证据均是在中枢神经系统存在感染或炎症等病理情况下获得的。本研究将灭活嗜水气单胞菌经蛛网膜下腔与肌肉两种途径接种健康犬，定期检测血清与脑脊液中的特异性抗体，旨在通过分析抗体出现的时间及抗体水平的高低来评定鞘内抗体的产生规律。　　12只2月龄土种幼犬，分成 ...