脂类生化技术

EXTRACTION AND ANALYSIS OF LIPIDS The lipophorin molecule that we isolated from locust hemolymph functions as a re-usable lipid shuttle. HDLp has a density of approx. 1.08 g/ml and contains less lipid ...

Hancock Laboratory MethodsDepartment of Microbiology and ImmunologyUniversity of British Columbia British Columbia Canada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=41 OBJECTIVE: To methylate ...

Hancock Laboratory MethodsDepartment of Microbiology and ImmunologyUniversity of British Columbia British Columbia Canada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=59 REAGENTS: CHCl3:MeOH (2 ...

Contributor: Suprya JayadevDate: August 21 1991 NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1µl of phenol red soluti ...

Contributor: Suprya JayadevDate: Mar. 11 1991 Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform. 2) Place a small portion of glass wool at the base of the column ...

Contributor: Suprya JayadevDate: November 4 1991 1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS. 3) Resuspend ...

Contributor: Suprya JayadevDate: Mar. 11 1991 I. Lower chloroform phase: 1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely but sufficiently to yield a fi ...

Contributor: Suprya JayadevDate: Nov. 10 1993 1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media conta ...

Contributor: Suprya JayadevDate: Mar. 11 1993 Synthesis reaction: 1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol. 2) Allow reaction to proceed at room ...

Contributor: Suprya JayadevDate: May 25 1993 Reagents: Lysis buffer25 mM Tris-HCl pH 7.45 mM EDTA1 mM ATP20 µg/ml CLAP1 mM PMSF Buffer A10 mM MgCl20.2 M Tris-HCl pH 7.40.2 % Triton X-100 Buffer ...

Contributor: Suprya JayadevDate: Oct. 20 1994 Reagents:2N methanolic NaOH 2N HCl-------------------------------------------------------------------------------- Protocol: Bligh & Dyer extraction 1 ...

OBJECTIVE: Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method. REAGENTS: Dioleoyl Phosphatidyl Choline Buffer of choice / dis ...

1) Prepare a C-18 reverse phase prep-sep cartridge by washing with: a) 10 ml of methanol b) 20 ml of chloroform-methanol (2:1) c) 10 ml of methanol d) 10 ml of methanol-1.6 M sodium acetate (1:1) 2) A ...

胆固醇是环戊烷多氢菲的衍生物，它不仅参与血浆蛋白的组成，而且也是细胞的必要结构成分，还可以转化成胆汁酸盐、肾上腺皮质激素和维生素D等。胆固醇在体内以游离胆固醇及胆固醇酯两种形式存在，统称总胆固醇。总胆固醇的测定有化学比色法和酶学方法两类。本实验采用前一方法。 胆固醇及其酯在硫酸作用下与邻苯二甲醛产生紫红色物质，此物质在550nm波长处有最大吸收，可用比色法作总胆固醇的定量测定。胆固醇含量在400m ...

一、目的 脂肪广泛存在于许多植物的种子和果实中，测定脂肪的含量，可以作为鉴别其品质优劣的一个指标。脂肪含量的测定有很多方法，如抽提法、酸水解法、比重法、折射法、电测和核磁共振法等。目前国内外普遍采用抽提法，其中索氏抽提法（Soxhlet extractor method）是公认的经典方法，也是我国粮油分折首选的标准方法。通过本实验的学习掌握索氏抽提法测定粗脂肪含量的原理和操作方法。 二、原&nbs ...

脂类测定标准化十分重要。心脑血管病的临床和流行病学研究，高脂血症的诊断或治疗过程中血脂变化的监测，以及冠心病危险性的估计都需要准确、精密和实验间可比的测定结果。 一、国外标准化工作的回顾和现状 早在1958年，美国疾病控制与预防中心(CDC)即提出脂类［胆固醇(chol)和甘油三酯(TG)］测定的标准化计划，并和世界卫生组织(WHO)合作，对美国和国际实验室进行脂类测定的质量评价。当时有800多 ...

脂蛋白和脂质测定方法学评价血浆脂蛋白和脂质测定是临床生化检验的常规测定项目，其临床意义主要是：早期发现与诊断高脂蛋白血症；协助诊断动脉粥样硬化症；评价动脉粥样硬化疾患如冠心病和脑梗塞等危险度；监测评价饮食与药物治疗效果。 一、血浆脂蛋白测定 1、超速离心分离纯化法超速离心法是根据血浆中各种脂蛋白的比重（密度）的差异，在强大离心力作用下进行分离纯化的一种方法。血浆在不同密度盐溶液中经过超速离心，各种 ...

脂蛋白和脂质测定方法学评价血浆脂蛋白和脂质测定是临床生化检验的常规测定项目，其临床意义主要是：早期发现与诊断高脂蛋白血症；协助诊断动脉粥样硬化症；评价动脉粥样硬化疾患如冠心病和脑梗塞等危险度；监测评价饮食与药物治疗效果。 详细内容请下载： ...

REFERENCES: Nikaido and Roseenberg (1983) J.Bact 153: 241-252; Yoshimura et al. (1983) J.Bact 258: 2308-2314. METHOD: 1、6.2 ?moles of lipid (ideally egg phophatidylcholine or dipalmitoyl phosphorylcho ...

Preparation of chambers: Immediately after use rinse with distilled water a couple of times and then with 70% or 100% ethanol (can repeat this rinsing) then invert the cells and let them air-dried. Th ...