细胞功能测定

The white adipose tissue stores energy in the form of triglycerides in time of nutritional excess and releases free fatty acids during food deprivation. The adipose tissue mass is determined by the balance between energy intake and expenditure. Alterations of this steady state can lead to ove ...

The ability of mouse embryonic stem (ES) cells to undergo differentiation in vitro complements their ability to contribute to numerous tissues in vivo and provides a unique model system for aspects of early mammalian development. ES cells are differentiated in two major ways: (i) unmanipul ...

Advances in our understanding of cardiomyocyte cell biology have been dependent largely upon the ability to generate primary cultures from enzymatically dispersed fetal, neonatal, or adult hearts. Primary cardiomyocyte cultures recapitulate many of the physiologic and mole ...

Embryonic stem (ES) cells have the potential to proliferate infinitely in vitro in an undifferentiated and pluripotent state, thereby maintaining a relatively normal and stable karyotype even with continual passaging. Remarkably, in vivo, ES cells can be reincorporated into normal ...

Embryonic stem (ES) cells are derived from undifferentiated cells present at the inner cell mass at the blastula stage of development (1,2). In the normal course of development, these cells give rise to the primitive ectoderm, which in turn gives rise to the ectoderm, mesoderm, and endoderm through ...

Embryonic stem (ES) cells are derived from the epiblast of mouse blastocyst. They can repopulate all cell lineages in vivo and can differentiate into a wide variety of cell types in vitro during embryoid body (EB) formation (1). ES cells have been shown to generate both neurones and glial cells (2,3). Dur ...

The epidermis is a stratified squamous epithelium that provides the protective layer of the skin. The mammalian epidermis is derived from embryonic ectoderm, and this layer eventually gives rise to a very early epithelial cell that further commits to become epidermal tissue (for review see ...

Hair is arguably the most crucial skin appendage that shapes one’s self-image and acceptance in our society. However, despite this critical function, the precise regulatory steps governing the formation of hair follicles remains poorly understood. It is known, though, that hair follicle ...

Embryonic stem (ES) cells were first isolated in the 1980s by several independent groups. (1–4). These investigators recognized the pluripotential nature of ES cells to differentiate into cell types of all three primary germ lineages. Gossler et al. (5) described the ability and advantages of ...

Melanocytes residing in the skin, inner ear, and uveal tract are derived from neural crest cells. They are highly dendritic, heavily pigmented, and are generally located in the epidermal basal cell layer of these areas, including hair follicles (1). The most convincing fact demonstrating the n ...

The use of mouse embryonic stem (ES) cells to alter the mouse genome has become a routine method to study gene function alongside classical transgenesis. Gene targeting by homologous recombination is the most widely used method of ES cell-mediated genome alterations. It allows the introduc ...

The zygote is a single cell, a fertilized egg, capable of giving rise to a complete organism. This is a truly remarkable transformation, with a single cell becoming trillions of cells or more, with a microscopic mass becoming in some cases tens or hundreds of kilograms, with a single amorphous cell making a ...

Methods to study the establishment and distribution of embryonic cell lineages have increased our understanding of the diverse events that comprise normal development. The resultant fate maps have also provided an important framework for systematically analyzing genotype-p ...

The pace of sequencing whole genomes by far exceeds our increase of knowledge on gene functions. Twenty-two genomes have been already completed and both the human and mouse genome are close to be completed as well. The number of sequences in the National Center for Biotechnology Information (NC ...

The gene trap approach, represented in Fig. 1, is based on the use of mouse embryonic stem (ES) cells and a class of vectors containing a splice-acceptor site upstream of the reporter and resistance genes, β-galactosidase (β-gal, lacZ) and neomycin resistance (neo), respectively. Integration of ...

Immunologic reagents such as antibodies can be valuable for identifying and isolating cells with particular characteristics. While antibodies derived from immunized animals are the most common immunologic reagents used for this purpose, “antibodies” selected by phage displ ...

Phage-mediated gene transfer offers an alternative method of introducing genes into specific cell types, including cell lines (1–4) and primary cell cultures (see Fig. 1). Recent studies demonstrate that filamentous bacteriophages can be engineered to transfer genes to mammalian c ...

Knowledge of the molecular and cellular events characterizing osteoblast development is growing as new markers, including important classes of regulatory molecules such as transcription factors (e.g., Cbfa-1 ), are elucidated. Nevertheless, a paucity of definitive and specific ...

During embryonic development, primitive ectoderm forms three primary germ layers, the mesoderm, the ectoderm, and the endoderm. These germ layers interact forming all the tissues and organs of the developing embryo. The influences controlling the transition of ectoderm to visceral a ...

Cellular gene expression changes during ontogenetic development of cell physiological activation-inhibtion and differentiation. Classical molecular assays like Southern, Northern, or Western blotting display only a few genes at once. The analysis of complex alterations ...