丁香实验_LOGO
登录
提问
我要登录
|免费注册

细胞功能测定

丁香实验推荐阅读
Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes

Determination of the flux of substances (ions or metabolites) into or out of cells is a recurrent issue in cell biology. This demands the incubation of cells in a medium followed by separation of the cells from this medium. To perform this type of experiment in brown, and especially in white, adipocytes, is ...

丁香实验推荐阅读
Culture of Adipose Tissue and Isolated Adipocytes

Adipose tissue (AT) function is regulated by both short-term and long-term mechanisms. Long-term adaptations to hormonal, nutritional, and developmental factors take place over a time course of hours to days. For example, chronic alterations in the hormonal milieu (analogous to those t ...

丁香实验推荐阅读
Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines

The possibility of maintaining viable cells in an incubator for prolonged periods, and studying dynamic changes in them in an environment that can be manipulated at will, is an attractive experimental approach. For studies of brown adipose tissue (BAT) recruitment, it provides a powerful a ...

丁香实验推荐阅读
Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity

Adipose tissues (ATs) were long considered as negligible and as simple filling tissues. The increase in knowledge concerning their role in energy balance and the increased occurrence of metabolic disorders, such as obesity and syndrome X, have focused the attention of the scientific comm ...

丁香实验推荐阅读
Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue

Preadipocyte cellular models have proven to be useful tools for the study of adipose cell development in vitro (1–5). Among these models are preadipocyte clonal lines from various origins and primary cultures of fibroblast-like adipose precursor cells present in the stromal-vascular ...

丁香实验推荐阅读
Cultures of Human Adipose Precursor Cells

Recently, there has been rapidly growing interest in cell culture models that allow the study of the adipose differentiation process in vitro, as well as the long-term regulation of fat cell metabolism in human adipose tissue (AT) material. Although valuable clonal preadipocyte cell lines ...

丁香实验推荐阅读
Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes

In addition to their metabolic function of storing triglycerides and releasing free fatty acids upon hormonal stimulation, adipocytes are now recognized as behaving as secretory cells. During the past 10 yr, an increasing number of peptidic and nonpeptidic compounds have been demons ...

丁香实验推荐阅读
Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences

Adipose tissue (AT) is more than a collection of adipocytes. It is a highly organized tissue in which different cell types interact, and in which a complex mix of hormones and substrates arriving in the plasma, together with neural input and the rate of blood flow (BF), all regulate metabolic activity. T ...

丁香实验推荐阅读
Measurement of Adipose Tissue Blood Flow

Adipose tissue (AT) is a well-vascularized tissue with a capillary density corresponding to that of skeletal muscle. The blood supply to the tissue is tightly coordinated with the tissue metabolism. AT blood flow (BF) is increased during exercise and starvation, i.e., in situations in which fat ...

丁香实验推荐阅读
Measurements of White Adipose Tissue Metabolism by Microdialysis Technique

The microdialysis method was introduced 25 yr ago to measure neurotransmitter concentrations in the brains of laboratory animals. For 10 yr, this technique has been adapted, in metabolic studies, to monitor the interstitial concentrations of small-mol-wt compounds present in the ext ...

丁香实验推荐阅读
Immunoassay Measurements of Leptin

Since the discovery of the circulating peptide hormone, leptin, the recognition of adipose tissue (AT) as an endocrine organ has added a new perspective to obesity research. Numerous studies have shown the involvement of leptin (expressed predominantly in AT, placenta, and stomach) in the p ...

丁香实验推荐阅读
Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue

Light microscopy (LM) allows study of the anatomy of the adipose organ. For instance, it permits investigation of its microscopic organization (possible lobular subdivision) and the arrangement in the tissue of vessels, nerves, and main cell types (1). The unilocular or multilocular orga ...

丁香实验推荐阅读
Confocal Microscopy of Adipocytes

The isolated rat adipose cell experimental system is the principal model for studies of the mechanism of insulin’s stimulatory action on glucose transport (reviewed in refs. 1–4). Despite the successful use of this preparation in biochemical studies, the unique structure of the adipose ce ...

丁香实验推荐阅读
Subcellular Fractionation of Adipocytes and 3T3-L1 Cells

For more than 30 yr, adipocytes have been an almost ideal model for the study of insulin action, because they can be prepared easily as a homogenous population of cells, they exhibit a marked hormonal response, and they can be fractionated into relatively pure membrane preparations. This subcell ...

丁香实验推荐阅读
Cellularity Measurements

Adipose tissue (AT) makes up 10–30% of body weight in normal-weight individuals, but its mass can vary from as little as 3–5% of body wt in lean, athletic subjects to 70% of body wt in very obese subjects.

丁香实验推荐阅读
Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies

Investigation of the in vivo regulation of gene expression in human subcutaneous white adipose tissue (WAT) relies on the ability to estimate the changes in specific mRNA levels in biopsies taken before and after a designed intervention (i.e., diet, exercise, hyperinsulinemic clamp, and so ...

丁香实验推荐阅读
Glycogen Synthase Activity in Adipose Tissue: Methods for Freeze-Clamping and Assay

One of the best markers of in vivo insulin-action at insulin sensitive tissues is an increase in glycogen synthase (GS) activity. GS is the rate-limiting enzyme of GS. The activity of glycogen synthase is increased by dephosphorylation, which converts the dependent form (dependent on glucose ...

丁香实验推荐阅读
Assays of Lipolytic Enzymes

Free fatty acids (FAs) derived from adipose tissue (AT) are, quantitatively, the most important fuel in mammals, and provide more than half of the caloric needs during periods when dietary substrates are lacking. Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in the lipo ...

丁香实验推荐阅读
Assays of Lipogenic Enzymes

The deposition of fat in adipose tissue (AT) is an important aspect of energy metabolism. AT fat originates either from ingested fatty acids (FAs) or from de novo synthesis. The synthesis of FAs is active in several tissues (liver, mammary gland, lung, intestine, brown and white ATs); however, in roden ...

丁香实验推荐阅读
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development

Osteoclasts are cells specialized to resorb bone (1,2). They originate from hematopoietic progenitors (3,4), which are widely distributed throughout the body. Osteoclast progenitors differentiate into cells that are positive for calcitonin receptor and tartrate-resistant ...

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序