细胞功能测定

PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays for flow chamber assays flow cytometry measurements etc.Materials ...

地高辛（DIG）产品选择指南――选择合适的标记方法标记DNA探针如果您有足够量的高纯度模板，建议您采用随机引物标记方法的DIG High Prime DNA Labeling and Detection Starter Kit I 和II。这两个试剂盒包含了标记、封闭、杂交和检测所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同：Starter Kit I采用了显 ...

小提.pdfplasmid.pdfVigoFect.pdfmatrigel.pdf ...

MATERIALSMedia: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated FBS IMDM + 20% heat-inactivated FBS Viral transduction: Iscove's + 20% heat inactivated FBS plus 100 ng/mL each of SCF IL ...

Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pay for both reserved time and the time actually used (w ...

NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells a hemocytometer and ImageJ software. The hemocytometer is not needed if you have already calibrated the areas ...

Materials 2x HEPES: 8 g NaCl 0.105g NaHPO4 6.5 g HEPES H2O to 500 mL pH 6.95 to 7.10 (try a range) 2M CaCl2: store in aliquots at -20°C DNA 4 mg/mL Procedure (The volumes can be scaled up as necessar ...

MaterialsWildtype MoMLV virus aliquot. Stored at -80ºC.Medium: DMEM + 10% FBSNIH 3T3 TK- cellsXC cellsPolybrene 1000x stock = 4 mg/mL sterile filtered.U.V. light source on a stand.ProcedureDay 0: T ...

1、将转染后的细胞按照一定的比例接种到6孔板中（一般选用1：10和1：100两个梯度），分别采用1200mg/ml，1000mg/ml和800mg/ml的G418进行筛选；以转染携带EGFP质粒载体的细胞作为阳性对照，以未转染质粒载体的细胞作为阴性对照；2、每3～4天换液一次；3、筛选20～30天，即可得到稳定表达细胞株；4、传代后，以一定的G418浓度维持培养； ...

The plasticity of differentiated adult cells could have a great therapeutic potential but at the same time it is characteristic of progression of serious pathological states such as cancer and fibrosi ...

1. 2 稳定转染细胞株的构建原理：稳定转染，就是转染的质粒DNA整合到宿主细胞染色体上，使宿主细胞可长期表达目的基因及蛋白。需要在瞬时转染基础上对靶细胞进行筛选或者应用高转染效率的病毒，根据不同基因载体中所含有的抗性标志选用相应的药物进行细胞传代，从而得到可稳定表达目的基因的细胞系。应用： 观察目的基因及其表达蛋白在某种细胞中的功能（稳定，可长期进行研究）一般流程：1） 筛选浓度测定： ...

OverviewCount the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cells. MaterialsLB agar plate LB media for dilution 8 ...

Cell cycle analysis of Escherichia coli cells C period = the time for a round of chromosome replication D period = the time between the end of a round of chromosome replication and cell division Deter ...

OverviewThis protocol is a variant of the Hanahan protocol using CCMB80 buffer for DH10B TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested o ...

MethodThaw TSS cells on ice. Add DNA pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl) be careful to mix the culture well. Diluting the p ...

CuratorsJingyang Chen Seattle Biomedical Research Institute 307 Westlake Ave N Suite 500 Seattle WA 98109 USA. jingyang.chen@sbri.org Anyone should feel free to add themselves as a curator for this co ...

IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr . It plays an important role in embryonic development maintenance of tissue homeostasis and pathology and therefore has b ...

INTRODUCTION Flow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone however cells in the quiescent G0 phase cannot be discriminated from cells ...

Protocols for Neural Cell Culture 2001 Humana.pdf美国进口bioworld抗体几大优势：1.2800多种美国进口一抗二抗，重组蛋白，在中国设立现货仓库，在3天内迅速到货。我们与顺丰快递合作的，运输没有问题2.质量保证，参考文献已突破400篇 ，很多权威客户在使用这个品牌抗体，如药物所，诺华，罗氏，中科院，复旦，交大，北大，清华，浙大等，完善的技 ...

A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch12 Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endosomes receive material from the plasma membrane by fus ...