细胞功能测定

DescriptionCell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure1. Carefully remove the growth media from the cell culture. 2. Add an equal volume of PBS and gently mix ...

Wash cells with PBS Fix cells in 0.2% glutaraldehyde for 5’ dilute this in PBS Wash cell with PBS Stain with X-gal solution (ingredients listed below) for 6 to 24 hours at 37°C Chemical Conc.Stocks ...

Outline:To measure NK cell killing suitable target cells are labeled with 51Cr washed and incubated together with the killer cells (and treatments). Large amounts of label is released into the superna ...

IntroductionLiving (metabolically active) cells reduce tetrazolium salts to colored formazan compounds; dead cells do not. Thus tetrazolium salt-based colorimetric assays detect viable cells exclusive ...

Materials Fibroblast cells in log phase growthCa Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chrom alum gelatin) and P ...

DescriptionCytotoxic activities of mNK cells are examined in 51Cr release assay from target cells ProcedureEffector cells (mNK cells) are seeded into round-bottom 96-well plates (Costar) in 100-?l ali ...

DescriptionIn vitro whole cell assay by Hypoxanthine uptake assay Procedure Hypoxanthine uptake assay (with respect to red blood cell cultures of P. falciparumDispense 25 ul complete RPMI in differen ...

Labelling:1) Seed cells in 6 well plates ( in normal growth media) such that there are 5 X 104 cells per well.2) Incubate cells 2 nights.3) Remove media wash cells one time with 1X PBS and add serum f ...

DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure1. Grow the cells in regular media to 70% confluence and ...

DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells noble agar assay can be utilized. Noble agar is used to ...

Note: All volumes are calculated to cater for four plates per point.Base Agar1. Melt 1% Agar (DNA grade) in microwave cool to 40� in a waterbath. Warm 2X RPMI + 20% FCS to 40� in waterbath. Allow at l ...

-Use sterile technique and sterile solutions throughout this method.- 1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation. ***It is important to make sure all strains ...

Preparation of cell lysate: 1. For cells in suspension grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1 K for 5 min) and obtain a concentration of cells a ...

Introduction Enzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies but subsequently the assay has been adapted for iden ...

Introduction A modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular antigens at t ...

OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens laminin and proteoglycans)but also matrix degra ...

(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab)1) Plate cells onto gelatinized plates (35 or 60 mm) without feeders and culture 24 hours. A 1:4 split is be ...

4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted to the endothelial lineage. In medaka embryos somi ...

Aspirate medium from a 6-well plate and wash with PBS Ca++/Mg++ free. Incubate for 30 min in 1.5 mg/ml Collagenase IV in DMEM:F12 at 37°C. Using a cell scraper remove the colonies and transfer to a 15 ...

This is an extract of the Amaxa Biosystems protocol Vs. 09-2005 optimized for use with the UC06 cell line. It is suggested that you try all 5 programs (A-23 A-27 A-13 A-12 B-16) with each Nucleofectio ...