细胞功能测定

1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml of your suspension of cells into 10 ml Tris or TD ...

Exercise 12.9 - Eukaryote Growth DynamicsLEVEL II Materials Suspension cultures set up from Exercise 12.8Sterile transfer pipettesMaterials for viability counting (Exercise 12.7) Procedure After 12 ho ...

OUTLINE We consider manual counts as less quick but more precize than authomatic (electronic) since in the latter case the counts are based on the size of particles so everything that has the same siz ...

Exercise 12.7 - Viability Cell CountMaterials Suspension culture of cellsSterile transfer pipettesStock 0.2% (w/v) Trypan blueHemacytometer and microscope Procedure Gently swirl a suspension culture t ...

Method: Preparation of Lymphocyte Cell Pellet for StorageJune 10 1990 Rosalie Veile Purpose: Following propagation to 1 X 108 cells lymphoblastoid cells are conveniently stored at -80 degrees C to pre ...

There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD or in rare cases with VE. This removes serum which contains trypsin inhibitors and lowers the c ...

There are two kinds of dishes used to grow tissue culture cells.Those that are designed for adherent cells have been treated chemically to promote cell adhesion and are called tissue culture dishes. R ...

Method: Lymphocyte TransformationMay 30 1990 Rosalie Veile Principle: Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated ...

Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci Donacki Date Added: Tue May 14 2002Date Modified: Tue Apr 27 2004Abstract: Detailed procedure for culture and subculture ce ...

Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30 1990 Rosalie Veile Purpose: To store cell lines in a form that will insure recovery with high viability.A culture in logari ...

It is well established with avian retroviruses that cells are most efficiently infected just after they are trypsinized. Trypsinization does two things. First it apparently exposes the receptor to whi ...

Method: Logging in Specimens and Record KeepingJune 10 1990 Rosalie Veile Purpose: To keep a written and computerized record of all cell lines the dates when cell lines were received and frozen freeze ...

No two cell lines behave exactly the same so you must learn the peculiarities or personality of each of the cell lines with which you work. Irrespective of cell type all cells are diluted or dispersed ...

Raft culture media (aka Green’s media)3 parts DMEM (including glutamine or glutamax)1 part Ham’s F125% FCSvarious supplements (not everybody uses the same supplements some don’t use any!) The followin ...

DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen coat cover slips (if using) or cell culture dishes: ...

Revised protocol for establishing a mammary cell line from tumorsDrench mouse in 70% ethanol pin and dissect openSterile dissection of tumor- leave some behind to use in histologyPlace tumor in smal ...

Exercise 12.10 - Establishment of a Primary CultureLEVEL III Materials Chick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors forceps and probesSterile petri platesPhosp ...

for axon tracing & cell culture studies in vitro using embyros age E11.5 � E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr before dissections:1. Prepare 1X Krebs (1000 ml) and ...

Author: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue Apr 27 2004MaterialsDMEM high glucose (Life Technologies Inc. #10313-021 or equivalent)Fetal Bovi ...

I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleukemia and leukemia.II. Culture Procedure:A bone marr ...