细胞功能测定

cell proliferation assaybefore start:thaw cells from liquid nitrogen grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until content gets cloudy and starts to turn yellow (usually w ...

Detection of BrdU Incorporation in DNA Synthesizing CellsNOTE: Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic and carcinogenic. 1.Pulse actively growing cells in ...

Determination of IC50Posted on Saturday January 17 2004 DescriptionIn vitro whole cell assay by Hypoxanthine uptake assay Procedure Hypoxanthine uptake assay (with respect to red blood cell cultures ...

Proliferation Assay: Thymidine incorporationContributor: Suprya JayadevDate: September 13 1994Labelling:1) Seed cells in 6 well plates ( in normal growth media) such that there are 5 X 104 cells per ...

When an irreplaceable culture becomes contaminated researchers may attempt to eliminate or control the contamination. First determine if the contamination is bacteria fungus mycoplasma or yeast. Isola ...

Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci Donacki Abstract: Detailed procedure for culture and subculture cell in flask and plates Purpose To describe the maintenan ...

IntroductionQuality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from ...

Preparation of low density collagenase-digested splenocytes Protocol 1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ ...

Blood Harvest・ Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail・ Pin animal to a board (use distal extremities) supine・ Use 70% alcohol to moisten the abdomen and lower ...

Defrosting Eukaryotic Cells Frozen In Liquid N2 Reagents / Solutions Frozen cell line. Growth media (e.g. RPMI1640) Foetal calf serum (FCS). ProtocolMake 20ml growth media/10% FCS for each vial of ce ...

Very useful Microsoft Access database program (486kb) for managing your cell lines stored in freezer. It's easy to use and has a very nice screen. 点击浏览该文件 ...

Trypan blue viability testOutline:Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than ...

Use of the Hemacytometer for the Determination of Cell Numbers Counting cells by the use of a hemacytometer is a convenient and practical method of determining cell numbers in the case that the Coulte ...

Eukaryote Growth DynamicsLEVEL II Materials Suspension cultures set up from Exercise 12.8Sterile transfer pipettesMaterials for viability counting (Exercise 12.7) Procedure After 12 hours aseptically ...

Use of a Hemacytometer A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the chamber floor. The counting ...

Viability Cell CountMaterials Suspension culture of cellsSterile transfer pipettesStock 0.2% (w/v) Trypan blueHemacytometer and microscope Procedure Gently swirl a suspension culture to distribute the ...

Load both sides of the hemacytometer with the cell suspension - covering both counting grids. Count the number of cells in 5 out of 9 squares from each counting grid (count a total of 10 squares). Div ...

Transfer of Eukaryote Suspension CulturesLEVEL II Materials Fibroblast suspension cultureTissue culture laminar flow hoodMedia appropriate to culture line usedDisposable pipettes (10 ml and 1.0 ml)Dis ...

Transfer of Eukaryote Suspension CulturesLEVEL II Materials Fibroblast suspension cultureTissue culture laminar flow hoodMedia appropriate to culture line usedDisposable pipettes (10 ml and 1.0 ml)Dis ...

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...