相关专题 无血清培养基产品选择指南 Serum-free media(SFM) 技术介绍 1、含血清成分培养体系的劣势 培养动物细胞,无论是为了增殖病毒,还是为了获得相关的生物制品,都力求使细胞大规模、高密度生长,并在高密度下维持高的细胞活性,简化下游纯化工艺,提高生物制品的安全性和产率,降低产品的成本,提高市场竞争力。但是常规细胞培养基的 ...
相关专题 胎牛血清产品选择指南 1、 如何储存和解冻血清才不会使产品质量受损? 将血清从冷冻箱取出后,先置于2~8℃冰箱使之融解,然后在室温下使之全融。但必须注意的是,融解过程中必须规则地摇晃均匀。 长时间储存在2-8℃时,血清中的各种蛋白和脂蛋白(如冷凝集素、纤维蛋白原、玻粘连蛋白等)可能聚集而形成沉淀或可见的混浊。因此,推荐在-20℃以下 ...
相关专题 胎牛血清产品选择指南 第一类,最高级别,是专门用于干细胞的特级胎牛血清 1、Hyclone的特级胎牛血清 目前,性能最好的血清,还是要说说Hyclone的特级胎牛血清,货号是SH30070.03,它是Hyclone的“招牌菜”。 Hyclone特级胎牛血清(Defined FBS),货号SH30070,是世界上唯一经过40纳米过滤的 ...
相关专题 本篇文章主要介绍了采用苏木素(Hematoxylin)-伊红(Eosin)染色法对细胞染色的实验原理、实验用品和具体实验步骤。 HE染色法 一、原理 苏木素(Hematoxylin)-伊红(Eosin)染色法,简称HE染色法。 HE染色法采用两种染料即碱性染料苏木素和酸性染料伊红分别于细胞核和细胞质发生作用,使细胞的微细结构 ...
相关专题 细菌是一种形态小、数量多的生物。在微生物实验中,我们经常需要对细菌进行计数,想必大家都数得很不耐烦吧。今天小编将为大家介绍几种细菌计数的实验方法。 细菌计数 1、计数器测定法 即用血细胞计数器进行计数。取一定体积的样品细胞悬液置于血细胞计数器的计数室内,用显微镜观察计数。由于计数室的容积是一定的(O.1mm3),因而根据计数 ...
相关专题 本月21日,首个“世界第一批经大规模平行测序技术的胚胎植入前胚胎遗传学诊断/筛查试管婴儿”现身长沙。此次科研成果的主要贡献者为人类干细胞国家工程研究中心主任、中南大学生殖与干细胞工程研究所所长卢光琇。 &NBSp; 卢光琇 这成为人类辅助生殖技术领域新的里程碑——积极性优生领域获重大突破,试管婴儿成功率将进一步提高,而婴儿出生缺 ...
相关专题 中国科学院青岛生物能源与过程研究所能源藻类资源团队在研究布朗葡萄藻培养效率方法上获得新进展,研究结果发现基于光能稀释的反应器结构并以生物膜形式贴壁培养将是解决布朗葡萄藻培养效率的重要手段。研究结果发表在"Bioresource Technology"期刊上。 布朗葡萄藻因可合成长链烃,且大部分分布在细胞外壁而易于提取等优点,被认为 ...
Matrigel invasion assays Posted on Saturday October 18 2003 DescriptionMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane componen ...
Cell Cycles - IntroductionThe onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes and the whitefish has o ...
Cell Cycles - IntroductionThe onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes and the whitefish has o ...
Advanced Methodologies in Flow CytometryAndrea Cossarizza EditorMaterials from a workshop held March 1997 in Modena Italy. All materials reproduced with permission. Introduction A. CossarizzaWorksh ...
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC PlatesRonald L. Schnaar~Professor Johns Hopkins University Medical School Baltimore Maryland 21205This procedure all ...
Immunofluorescent Localization of TubulinLEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute) in a 50:50 volume mixtu ...
Immunofluorescent Localization of TubulinLEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute) in a 50:50 volume mixtu ...
Isolation of Actin and Myosin FilamentsLEVEL III Materials Relaxing Solution0.1 M KCl0.001 M MgCl5 mM ATP0.016 M NaHPONaHPOAdjust pH to 7.30.05 M Sodium phosphate buffer pH 7.00.001 M EDTA (Ethylene d ...
Isolation of Microtubules (Bovine Brain)LEVEL II Materials Freshly removed bovine brain 2Wire sieve (tea strainer)Microtubule buffer (MT buffer)0.1 M MES (2-(N-Morphilino)ethanesulfonic acid)1 mM EGTA ...
Em observations of microsomesLEVEL I Figure 7.3 Isolated microsomes Figure 7.4 TEM of hepatocyte MATERIALS 1% Glutaraldehye (GTA)1% Osmium tetroxideEpoxy or vinyl resin for TEMTEM photomicrograph of ...
Em observations of microsomesLEVEL I Figure 7.3 Isolated microsomes Figure 7.4 TEM of hepatocyte MATERIALS 1% Glutaraldehye (GTA)1% Osmium tetroxideEpoxy or vinyl resin for TEMTEM photomicrograph of ...
Programmed Cell Death In Situ Detection Using Terminal deoxynucleotidyl Transferase (TdT) Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecti ...
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. Wilcox andJosé C. Rodriguez - Emory University - April 1996Protocol for Fro ...