细胞功能测定

细胞的分裂指数一、原理体外培养细胞生长、分裂繁殖的能力，可用分裂指数来表示。它与生长曲线有一定的联系，如随着分裂指数的不断提高，细胞也就进入了指数生长期。分裂指数指细胞群体中分裂细胞所占的百分比，它是测定细胞周期的一个重要指标，也是不同实验研究选择细胞的重要依据。二、仪器、用品与试剂1、仪器与用品：CO2培养箱、普通显微镜、细胞培养血、盖玻片、吸管2、试剂：培养液、胰酶、甲醇、冰醋酸、Giemsa ...

一、配制用水培养基的大部分是水，所以水的质量直接影响培养基的质量。按Waymouth标准，水的电阻应为200万欧姆，一般实验室里以玻璃蒸馏器制备的双蒸水或三蒸水可以符合使用条件。二、培养基培养基有天然、人工两种。一般实验室使用的是RPMI-1640粉末培养基，以双蒸或三蒸水配制。NaHCO3（或HCl）调pH至7.2～7.4，若pH值超过7.6或远低于6.8，则大多数细胞不能生长。RPMI-164 ...

PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxis is the ability of a protein to ...

一、纺锤体阻断剂（Spindle inhibitor）在有丝分裂过程中，随着纺锤丝的形成，染色体被牵引到一起难以观察其形态。纺锤体的形成在于细胞质和纺锤体成分的粘度之间的平衡，因此，改变细胞质的粘度，即可破坏纺锤体形成，从而使得染色体均匀散开，且染色体缢痕区更为清楚。在培养中使用的纺锤体阻断剂为秋水仙素，在终止培养前加入适量秋水仙素，使正在分裂的细胞停留在中期，以获得大量分裂相供分析之用。秋水仙素 ...

PurposeThe main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule on their surface and their receptor either directl ...

PurposeMaterials10ml 6% dextran + 7ml citrate/citric acid Dextran: T500 -- 6g+100ml PBS Citrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 1077 18 ml cold H ...

IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows following dynamic cellular processes as they unfold "in ...

IntroductionMulticolour 3D-FISH in combination with confocal microscopy 3D image reconstruction and quantitative image analysis is an efficient tool for the analysis of the 3D genome structure and of ...

地高辛（DIG）产品选择指南――选择合适的标记方法标记DNA探针如果您有足够量的高纯度模板，建议您采用随机引物标记方法的DIG High Prime DNA Labeling and Detection Starter Kit I 和II。这两个试剂盒包含了标记、封闭、杂交和检测所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同：Starter Kit I采用了显 ...

Materials:Colcemid (GIBCO: cat #: 15210-040)Hypotonic solution (pre-warmed 37C): 1:1 0.4% KCl + 0.4% Sodium citrateFixative: 3:1 MeOH + Acetic acid (e.g. 15 mL MeOH + 5 mL Acetic acid)Pasteu ...

（一）实验前应明确的问题1.选择适当的细胞接种浓度。一般情况下，96孔培养板的一内贴壁细胞长满时约有105个细胞。但由于不同细胞贴壁后面积差异很大，因此，在进行MTT试验前，要进行预实验检测其贴壁率、倍增时间以及不同接种细胞数条件下的生长曲线，确定试验中每孔的接种细胞数和培养时间，以保证培养终止致细胞过满。这样，才能保证MTT结晶形成酌量与细胞数呈的线性关系。否则细胞数太多敏感性降低，太少观察 ...

Materials: RPMI 1640 medium fetal calf serum (FCS) 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents Best.-Nr. 295892) cell cuture flask Phythemaglutinin PHA-L (Seromed M 5030) CO2 cell cul ...

Materials 4 mice from each genotype 4 Ly5 mice Buckets with wet ice 3x Bucket with dry ice 1x Dewar flask with liquid nitrogen 100 mL beakers with 95% ethanol 2x 100 mL beaker with dd. H2O 2x Sterile ...

NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells a hemocytometer and ImageJ software. The hemocytometer is not needed if you have already calibrated the areas ...

MaterialsWildtype MoMLV virus aliquot. Stored at -80ºC.Medium: DMEM + 10% FBSNIH 3T3 TK- cellsXC cellsPolybrene 1000x stock = 4 mg/mL sterile filtered.U.V. light source on a stand.ProcedureDay 0: T ...

Material:Packaging Cells e.g. Phoenix cells (an adenovirus Ad5-transformed human embryonic kidney cell line 293T transfected with two MoMLV packaging gene constructs: CMV-Env-PolyA and RSV-Gag/Pol-Tyt ...

（一）原理 混合单个核细胞悬液在通过尼龙毛柱时，B细胞、浆细胞、单核细胞和一些辅助细胞被选择性粘附于尼龙毛上，而多数T细胞则通过尼龙毛柱，这是获得富含T细胞群的有效方法。（二）材料及试剂1．尼龙毛（Nylon Wool Fiber）。2．烧杯、铝箔、漏斗、一次性手套等。3．装填尼龙毛柱，一次性注射器。4．取自然沉降的上层血浆，过聚蔗糖―泛影葡胺分层液后，获取的血浆和分层液之间的单个核细胞层。（三 ...

1、将转染后的细胞按照一定的比例接种到6孔板中（一般选用1：10和1：100两个梯度），分别采用1200mg/ml，1000mg/ml和800mg/ml的G418进行筛选；以转染携带EGFP质粒载体的细胞作为阳性对照，以未转染质粒载体的细胞作为阴性对照；2、每3～4天换液一次；3、筛选20～30天，即可得到稳定表达细胞株；4、传代后，以一定的G418浓度维持培养； ...

Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular ti ...

The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with e ...