细胞功能测定

This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program developed by Becton Dickinson. The problem that follow are presented in increasing degress of co ...

It is well established with avian retroviruses that cells are most efficiently infected just after they are trypsinized. Trypsinization does two things. First it apparently exposes the receptor to whi ...

No two cell lines behave exactly the same so you must learn the peculiarities or personality of each of the cell lines with which you work. Irrespective of cell type all cells are diluted or dispersed ...

DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen coat cover slips (if using) or cell culture dishes: ...

-embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. -metanephroi and associated ureteric buds are microdissected and placed in holding medium (L15 medium supplemented with ...

In vitro culture of embryonic lungsfrom Hogan Lab Isolation of Lung Bud Endoderm What you need: E11-12 mouse embryos DMEM with 5% fetal bovine serum petri dishes for dissections and washes Tyrode-Ring ...

-embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c. -limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ serum extender) (all media is ...

Revised protocol for establishing a mammary cell line from tumorsDrench mouse in 70% ethanol pin and dissect openSterile dissection of tumor- leave some behind to use in histologyPlace tumor in smal ...

I. Purpose:Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities.II. Culture Procedure:A. Aseptic technique must be used when setting up the cultures under ...

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...

ReagentsMedium:500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029)55 ml FCS (10 %)2.8 ml Gentamycin Solution (Sigma G-1272 10 ml)) TrypsinTrypsin (10x) lyophilised (Gibco 25095-019) SolutionVer ...

MN in Human Lymphocytes (method description)Lymphocyte isolationLymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted 1:1 with phosphate buffered saline and layered ont ...

Please note: lately (2005 - present) we normally culture PtK1 cells in F-12 media but they also grow in other types of media as described below. Mixing F-12 (HAM) Mediundefined ~undefinedSigma stopped making the po ...

ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type 5 DNA and Epstein-Barr virus nuclear antigen 1 al ...

AimIn general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ...

AimFor the majority of manipulations using cell cultures such as transfections cell fusion techniques cryopreservation and subculture routines it is necessary to quantify the number of cells prior to ...

The kitchen makes Tris TD Tryp/TD PBS and VE.Tris is a fairly complex Tris-buffered physiological saline solution. It is used to wash cells and is preferable to TD unless you need a Ca++/Mg++ free sol ...

We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result most of our incub ...

The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes and the whitefish has one of the clearest views o ...

BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-binding ...