细胞功能测定

The idea of antiangiogenesis as a therapeutic strategy has been around for several decades (1). Vigorously pursued as a novel anticancer strategy (reviewed in (2–6), it is now widely considered to be a promising approach to the treatment of a range of pathologies of which uncontrolled vascular p ...

Although it has been recognized for many centuries that neoplastic tissue is more vascular than its normal counterpart, it is only since Folkmans’ hypothesis on antiangiogenesis (1) that a more quantitative method for measuring angiogenesis in tissue sections has been pursued. Folkman ...

Angiogenesis, the process of new blood vessels forming from preexisting vessels, is an important feature in developmental processes, wound healing, and pathologic conditions such as cancer and vascular diseases. Owing to the importance of angiogenesis, a relatively simple and rapid ...

Recently, the acceleration (and retardation) of blood vessel growth has been an increasingly frequent subject of study. With its potential application to a wide range of clinical disease processes, investigation certainly remains essential and promising. While in vitro investig ...

The aim of our research was to develop a quantitative assay for angiogenesis in mammals, especially the mouse. This is a convenient experimental animal because of its small size, which allows compact housing and experimentation with angiogenic factors or inhibitors in limited supply. Mou ...

The process of capillary growth, angiogenesis, is an integral part of wound healing and repair mechanisms. When it occurs during these conditions, it is tightly controlled and strictly delimited. However, in tumor growth and in a variety of vascular diseases, unrestrained angiogenesis c ...

Since 1924, when the first transparent chamber model in animals was introduced by Sandison (1), many other chamber models have been described in the literature for studying angiogenesis and microcirculation in a wide variety of neoplastic and nonneoplastic tissues by means of intravit ...

It is becoming increasingly clear that the process of angiogenesis is important for tumor growth and metastasis and as such provides an exploitable target for therapeutic intervention. Consequently a number of useful model systems has been developed to investigate the angiogenic pr ...

The study of the angiogenic process and the search for novel therapeutic agents to inhibit, or stimulate, angiogenesis has employed a wide range of in vivo ’angiogenesis’ assays (reviewed in 1–3). These differ greatly in their difficulty, quantitative nature, rapidity, and cost. The classic ...

The inner lining of blood vessels, the endothelium, consists of a monolayer of endothelial cells (ECs), that present a free luminal surface and attach on their abluminal side to the underlying basement membrane (apart from a minimal amount of cell-cell overlap). A great deal of heterogeneity ex ...

Angiogenesis is a complex morphogenetic process involving the coordinate migration of several cell types, including endothelial cells (EC), pericytes, and stromal fibroblasts (1–4). Angiogenesis is regulated by interactions between cells, soluble factors, and extracellu ...

Angiogenesis is a necessary component of normal tissue repair, tumor growth and dissemination, and a wide variety of other inflammatory and pathological processes as well, including diabetic retinopathy, rheumatoid arthritis, and psoriasis. Consequently, the last two decades h ...

A variety of in vivo and in vitro methods have been used to study angiogenesis, the process of blood vessel formation. Two widely accepted but technically difficult assays include the cornea implant assay and the chick chorioallantoic membrane assay. The cornea assay requires special equi ...

Human endothelial cells (EC) can be readily prepared from large vessels such as the aorta, and umbilical (1) and saphenous veins. ECs derived from these vessels have proven an abundant, convenient, and useful tool for the investigation of many aspects of endothelial biology. However, the major ...

The endothelium is involved in a number of normal physiological processes (regulating circulating levels of vasoactive agents, blood/gas exchange, regulating cellular traffic between intavascular and extravascular compartments of tissues, maintenance of the blood brain ...

The fusion of postmitotic mononucleated myoblasts to form syncytial myofibers is a critical step in the formation of skeletal muscle. Myoblast fusion occurs both during development and throughout adulthood, as skeletal muscle growth and regeneration require the accumulation of a ...

Successful completion of fertilization in mammals is dependent on three membrane fusion events. These are (1) the acrosome reaction of sperm, (2) the fusion of sperm and egg plasma membranes to form a zygote, and (3) the cortical reaction of fertilized eggs. Extensive research into the molecular ...

It has been known for more than 150 years that syncytial fusion is a normal feature in biological systems. In humans there are two larger syncytial tissues: skeletal muscles fibers and placental syncytiotrophoblast. Other fusion events take place as well from fertilization of the oocyte to in ...

The process of creating a single cell from two progenitor cells requires molecular precision to coordinate the events leading to cytoplasmic continuity while preventing lethal cell lysis. Cell fusion characteristically involves the mobilization of fundamental processes, in ...

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understo ...