细胞功能测定

Endothelial cells line the entire circulatory system and form the interface between the blood vessel intima and the circulating red blood cells. Endothelial cells are crucial to the proper function of the circulatory system and tissue viability, including their roles in coagulation, f ...

Human embryonic stem cells (hESCs) represent a promising source of cells for modern regenerative medicine due to their highly self-renewal capability and pluripotency to differentiate into almost all cell types from three different germ layers. Recent advances in the stem-cell field ...

Conventional methods for vascular endothelial differentiation of human embryonic stem (hES) cells had suffered from subculture incompetence of the final products due to dominant expansion of contaminating pericytic components. We have overcome this problem by adding a “hemat ...

Pluripotent human embryonic stem cells (hESCs) comprise of cells from all three germ layers in vivo. They have been proved to differentiate in vitro into a variety of cell lineages. Endothelial cells derived from hESCs could potentially contribute to cellular treatment of vascular disea ...

Induced pluripotent stem (iPS) cells have been generated through nuclear reprogramming of somatic cells via retrovirus- or lentivirus-mediated transduction of exogenous reprogramming factors OCT3/4, SOX2, KLF4, and c-MYC. The extraembryonic amnion is considered to be a promis ...

The reprogramming of human somatic cells to induced pluripotent stem (iPS) cells has offered the �opportunity to derive patient-specific cells with embryonic stem cell (ESC) properties. Human iPS cells demonstrate the ability to self-renew and to differentiate into any cell type of the b ...

Induced pluripotent stem cells (iPSCs) provide an invaluable resource for regenerative medicine to repair tissues damaged through disease or injury. Although human iPSCs have been generated using different type of somatic cells, such as skin fibroblasts and keratinocytes, neural ...

Human induced pluripotent stem cells (hiPSCs) provide a unique experimental reagent for dissecting the complex transcriptional, regulatory, and epigenetic mechanisms of pluripotency, as well as for studying normal and diseased human development. However, the utility of curre ...

The ability of somatic cells to be induced to pluripotency by ectopic expression of defined transcription factors has altered the course of research in developmental biology and regenerative medicine. Somatic cell reprogramming has now been performed with numerous somatic sourc ...

Mesenchymal stem cells (MSCs) are adult stem cells which show differentiation capabilities toward various cell lineages, and the MSCs can be cryopreserved for a long term without noticeable loss of these capabilities. We have used bone marrow–derived MSCs for treatments of patients with ...

One of the possible applications of human induced pluripotent stem (iPS) cells is its usage for transplantation (Takahashi K et al. Cell 131:861–872, 2007; Yu J et al. Science 318:1917–1920, 2007; Park IH et al. Nature 451:141–146, 2008; Lowry WE et al. Proc Natl Acad Sci USA 105:2883–2888, 2008; Morita S et al. Gene ...

Human induced pluripotent stem (iPS) cells have been derived mostly from cells originating from �mesoderm and in a few cases from ectoderm. This has prevented comprehensive comparative investigations of the quality of human iPS cells of different origins. We have recently reported for t ...

Substantial progress has been made in somatic cell reprogramming through ectopic expression of four transcription factors to yield induced pluripotent stem (iPS) cells. We have used the robust viral-based modification procedure to generate iPS cells from human umbilical vein end ...

This chapter describes a robust method for the generation of iPS cells from non-cultured human cord blood cells. We describe the preparation of the CD34+ fraction from cord blood mononuclear cells, the protocols to determine the pluripotency of the reprogrammed cells, the culture conditi ...

Until now, it has been problematic to obtain high survival rates after thawing of human embryonic stem cells (hESCs) and later also induced pluripotent stem cells (iPSCs). Already in 1994, Freshner and �coworkers established the conventional slow freezing/rapid thawing methods by using a ...

In the mouse, embryonic germ (EG) cell lines can be generated from primordial germ cells (PGCs) �recovered from the genital ridge of the developing fetus. These EG cells have the capacity of self-renewal, are pluripotent, and have the potential to differentiate to other cell types of the body simil ...

The hallmark of pluripotent stem cells is their nearly unlimited self-renewal capacity, and their potential to differentiate into a diverse range of specialized cell types. These unique properties make stem cells important research tools, in vitro models for pharmaceutical testin ...

Although embryonic stem (ES) cells have the regenerative capability of producing any tissue in our body, the development of efficient methods to generate a large number of ES cells with high purity has still remained a technical challenge before they can be used routinely in clinical trials. In t ...

We have developed a simple yet highly reproducible technique of upscaling single cell–inoculated human embryonic stem cells (hESCs) in suspension cultures, using defined, nonconditioned media. Mass expansion of hESC was readily achieved by serially upscaling 2-ml static cultur ...

The culture of human pluripotent stem cells under defined conditions most commonly relies on the use of Matrigel as a substrate upon which the cells remain in an undifferentiated state. Matrigel is a complex mixture of extracellular matrices and growth factors derived from mouse Engelbre ...