前沿科技及其它

Anoikis, a form of programmed cell death that occurs due to cell detachment from the extracellular matrix, is a critical mechanism in preventing ectopic cell growth. Acquisition of resistance to anoikis is a prerequisite for epithelial ovarian cancer cells to survive in ascitic fluids bef ...

The concept of cancer stem cells (CSCs) provides a new paradigm for understanding cancer biology. Cancer stem cells are defined as a minority of cancer cells with stem cell properties responsible for maintenance and growth of tumors. The targeting of CSCs is a potential therapeutic strategy to ...

We have refined the technique for isolating and propagating cultures of primary epithelial ovarian cancer (EOC) cells derived from solid tumors and ascites. Both protocols involve a simple yet rapid method for the growth and propagation of EOC tumor and ascites cells in a basal culture medium w ...

Specific biological properties of ovarian cancer cells can be modeled and studied using in vitro experiments. Any experimental setting can closely reflect some aspects of the native conditions; however, parameters that differ from in vivo aspects must be considered. Familiarity with ...

Changes in protein glycosylation are a hallmark of most types of cancer including ovarian carcinoma. The structural elucidation of glycans is technically challenging and it requires complementary chromatographic and spectroscopic techniques among others. Here, we describe ...

Reverse phase protein array (RPPA) is a very suitable technique to analyze large numbers of proteins in small samples like for example tumor biopsies. Beside their small size another major hindrance for the analysis of proteins from biopsies is the extraction of proteins from formalin-fix ...

Herein, we have utilized two cellular models of epithelial ovarian cancer (EOC), where transfer of normal chromosome 18 material into the EOC cell lines TOV-112D and TOV-21G induced in vitro and in vivo suppression of tumorigenic phenotype in derived hybrid clones. Two-dimensional-liqu ...

The revised version of cancer hallmarks, depicting the biological properties acquired during tumor development and progression, includes the capability to modify or reprogram cellular metabolism. High-resolution multinuclear magnetic resonance spectroscopy (MRS) p ...

Most of the research on tumor cell metabolism has focused on glucose utilization. However, when glucose is limited, solid tumors are forced to catabolize alternative substrates such as fatty acids and amino acids as an energy source. Measuring these alternations in tumor cell metabolism en ...

Ovarian cancer possesses metabolic properties typical for any malignancy as well as some specific characteristics. Most of the methodological approach to study metabolism and molecular composition of the living cells are suitable for ovarian cancer research, however, might req ...

MicroRNAs (miRNAs) and genes work cooperatively to form the kernel part of gene regulatory system and affect many crucial biological processes. However, the detailed combinatorial roles of most miRNAs and genes in cellular processes and diseases are still unclear. The huge amount of dive ...

The detailed analysis of noncoding RNA is an upcoming necessity due to a plethora of recently identified components of this class of molecules. The investigation of their structure, directionality, intracellular localization, interaction with other cellular elements is useful to ...

The evaluation of microRNA profiles has represented one of the first approaches to investigate the aberrant microRNA expression in different human cancers, providing the first experimental evidence of the involvement of these small noncoding RNAs in the tumorigenic process. Curr ...

Alternative splicing is an essential process for the generation of protein diversity. The physiological role, cellular localization, and abundance of splice variant products compared to the wild-type protein may be completely different. This is illustrated by the five splice vari ...

The next-generation sequencing technology allows identification and cataloging of almost all mRNAs, even those with only one or a few transcripts per cell. To understand the chemotherapy response program in ovarian cancer cells at deep transcript sequencing levels, we applied two nex ...

Transcriptome profiling is a powerful method for monitoring genes and their expression levels under a variety of conditions. Completion of the human genome and advances in high-throughput gene microarray instrumentation enables one to collect large amounts of data in a relatively sh ...

Development of ovarian cancer is known to be associated with alterations in the expression of cellular RNAs. Most of the clinical and biological characteristics of ovarian cancer have been correlated with significant changes in the expression of a subset of genes. Over the last few years, con ...

Bisulfite pyrosequencing is a sequencing-by-synthesis method used to quantitatively determine the methylation of individual CG cytosines from PCR amplicons of a region up to 115 bases in length. The procedure relies on prior bisulfite conversion of all potentially methylated CG cy ...

Bisulfite sequencing of cloned alleles is a widely used method for capturing the methylation profiles of single alleles. This method combines PCR amplification of the bisulfite-modified DNA with the subcloning of the amplicons into plasmids followed by transformation into bacter ...

Epigenetic modifications, including DNA methylation, are critically important mediators of normal cell function over the course of our lives. These modifications therefore also can play prominent roles in the development of disorders and diseases, including ovarian cancer. Ge ...