前沿科技及其它

The human MLL gene is one of the most promiscuous recombination hot spots of our genome with regard to the onset of malignant diseases. With the exception of gene internal partial-tandem duplications involving several exons located in the 5′-end of MLL, all recombination events occur in a small ge ...

Fluorescence in situ hybridization (FISH) provides one of the few ways of analysing the genotype of individual cells, an important consideration for mixed cell populations such as those found in leukaemia. A more sophisticated variation combines fluorescence immunophenotyping ...

Cytogenetics is integral to the diagnosis of childhood leukaemia, particularly in relation to the risk stratification of patients for treatment. Fluorescence in situ hybridization (FISH) has become an important complementary technique, expanding chromosomal analysis into ...

Many of the acquired genetic changes that contribute to the molecular pathogenesis of leukemia are well characterized. The relative simplicity of the tumor genetics of the common subtypes of leukemia and the availability of archived material in the form of archived neonatal blood spots ( ...

Disruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Many of the advances in the understanding of these mechanisms are attributable to the successful development of chromatin immunoprecipitation (ChIP) for in vivo detection of histone mod ...

Aberrant distribution of cytosine methylation in cancer has been linked to deregulation of gene expression and genomic instability. DNA methylation changes in cancer include both hyper and hypomethylation, and the precise localization of these changes is directly related to the im ...

Acute leukemia is an aggressive form of hematological malignancy, which is characterized and classified into different subtypes according to the morphology and immunophenotype of the leukemic blasts. However in the past decade, it became clear that it is the genetic makeup and probably ...

In mammals the HOX network consists of 39 genes which encode master regulators of developmental processes including hematopoiesis. Many of the chromosomal translocations associated with acute leukemias involve HOX genes directly or some of their regulatory factors, e.g., mixed li ...

The two-hybrid system is a genetic method to search for and to identify direct interaction partners of a protein of interest. This method is instrumental to elucidate the transformation mechanism of several oncogenes that play a role in childhood leukaemia. With respect to mixed lineage leu ...

Hematopoietic stem cells are at the foundation of the blood system. Their study is not only relevant to the understanding of the basic cellular mechanisms of self-renewal, lineage commitment, and differentiation, but they have also been the target of intense clinical research into the caus ...

Embryonic Stem (ES) are pluripotent cells derived from the inner cell mass of blastocysts. ES cells differentiate in vitro into all kind of cells and the development of endothelial and hematopoietic cells from mouse ES cells has been especially established. As such, the in vitro differentiat ...

The study of key mechanisms and molecules involved in the regulation of hematopoiesis in mouse models has been greatly facilitated by multi-parameter flow cytometry. Subpopulations of hematopoietic stem and progenitor cells can be identified and characterized using this techni ...

With the emergence of the concept of the leukemia stem cell, assays to study them remain pivotal in understanding (leukemic) stem cell biology. Although the in vivo NOD-SCID xenotransplantation model is still the favored model of choice in most cases, this system has some limitations as well, such ...

The use of primary human cells to model cancer initiation and progression is now within the grasp of investigators. It has been nearly a decade since the first defined genetic elements were introduced into primary human epithelial and fibroblast cells to model oncogenesis. This approach has n ...

The xenotransplantation model has been instrumental for the identification and characterization of human leukemic stem cells. In this chapter we will discuss the development of the immunodeficient model in the understanding of leukemogenesis, describe the different models of i ...

Characterization of gene expression programs and pathways important for normal and cancer stem cells has become an active area of investigation. Microarray analysis of various cell populations provides an opportunity to assess genomewide expression programs to define cellul ...

Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by th ...

Direct analysis of T-cells of defined specificity and phenotype, without in vitro manipulation that accompanies limiting dilution analysis or other restimulation protocols for evaluating precursor frequency, provides the most accurate representation possible of in vivo e ...

Melanoma cells are considered to be immunogenic because they express melanoma-associated antigens that are recognized by autologous T-cells. The role of T-lymphocytes in the host’s immune response to cancer in general and to melanoma in particular has been studied intensively during ...

Melanomas are most frequently infiltrated by actively proliferating T-lymphocytes (1). Some of these T-cells are cytolytic and recognize peptide antigens derived from melanoma-specific antigens (2). However, with the noteworthy exception of rare immune-mediated, sponaneo ...